Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification and Characterization of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Kimchi

  • Lee, Hun-Joo (Cellular Response Modifier Research Unit Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Chan-Sun (Cellular Response Modifier Research Unit Korea Research Institute of Bioscience and Biotechnology) ;
  • Joo, Yun-Jung (Cellular Response Modifier Research Unit Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Seung-Ho (Protein Function Research Unit Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoon, Jung-Hoon (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Yong-Ha (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hwang, In-Kyeong (Department of Food and Nutrition, Seoul National University) ;
  • Ahn, Jong-Seog (Cellular Response Modifier Research Unit Korea Research Institute of Bioscience and Biotechnology) ;
  • Mheen, Tae-Ick (Cellular Response Modifier Research Unit Korea Research Institute of Bioscience and Biotechnology)
  • Published : 1999.06.01
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Abstract

Lactic acid bacteria were isolated from Kimchi and screened for bacteriocin. A total of 99 strains showed antimicrobial activity when grown on solid media, yet only 10 showed antimicrobial activity in liquid media. Strain H-559, identified as Lactococcus lactis subsp. lactis, exhibited the strongest inhibitory activity and was active against pathogenic bacteria including Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus as well as other lactic acid bacteria. The antimicrobial substance produced by L. lactis subsp. lactis H-559 was confirmed to be a bacteriocin by the treatment of $\alpha$-chymotrypsin, and protease type Ⅸ and ⅩIV. The bacteriocin activity remained stable between pH 2.0 and pH 11.0 and during heating for 10 min at $100^{\circ}C$. The bacteriocin production started in the exponential phase and stopped in the stationary phase. L. lactis subsp. lactis H-559 showed the highest bacteriocin activity at a culture temperature of $25^{\circ}C$, and an inverse relationship between the bacteriocin productivity and mean growth rate at different culture temperatures was observed. The mean growth rate and bacteriocin productivity of L. lactis subsp. lactis H-559 increased as the initial pH of the media increased.

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References

  1. J. Appl. Microbiol. v.84 Purification and characterization of thermophililn T, a novel bacteriocin produced by Streptococcus thernophillus ACA-DC 0040 Aktypis, A.;G. Kalantzopoulos;J. H. Huis in't Veld;B. ten Brink
  2. Antimicrob. Agents Chemother v.26 Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B Barefoot, S. F.;T. R. Klaenhammer
  3. J. Diary Sci. v.71 Inhibitory action of nisin against Listeria monocytogenes Benkerroum, N.;W. E. Sandine
  4. Appl. Environ. Microbiol. v.57 Influence on growth conditions on the production of a bacteriocin, pediocin, AcH, by Pediococcus acidilactici H Biswas, S. R.;P. Ray;M. C. Johnson;B. Ray
  5. J. Microbiol. Biotechnol. v.8 Inhibition of latic acid bacteria in Kimchi fermentation by Nisin Choi, M. H.;Y. H. Park
  6. J. Microbiol. Biotechnol. v.7 Plasmid-associated bacteriocin production by Leuconostoc sp. LAB145-3A isolated from Kimchi Choi, Y. O.;C. Ahn
  7. Manual of the Identification of Medical Bacteria(2nd ed) Cowan, N. R.;K. J. Steel
  8. J. Gen. Microbiol. v.138 Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations De Vuyst, L.;E. J. Vandamme
  9. Bacteriocins of Lactic Acid Bacteria Antimicrobial potential of lactic acid bacteria De Vuyst, L.;E. J. Vandamme;L. De Vuyst(ed.);E. J. Vandamme(ed.)
  10. Microbiology v.142 Primary metabolite kinetics of bacteriocin biosynthesis by Lactobacillus amylovorus and evidence for stimulation of bacteriocin production under unfavourable growth conditions De Vuyst , L.;R. Callewaert;K. Crabb
  11. Food Technol. v.44 Nisin and its uses as a food preservative Delves-Broughton, J.
  12. PHYLIP:Phylogenetic Inference Package(version 3.5) Felsenstein, J.
  13. Bergey's Manual of Determinative Bacteriology(9th ed.) Halt, J.G.;N. R. Krieg;P. H. A. Sneath;J. T. Staley;S. T. Williams
  14. J. Food. Prot. v.52 Antimicrobial activity of lactic acid bacteria against Listeria monocytogenes Harris, L. J.;M. A. Daeschel;M. E. Stiles;T. R. Klaenhammer
  15. Appl. Microbiol. Biotechnol. v.32 Comparative studies on the fermentative production of lantibiotics by Staphylococci Horner, T.;V. Ungermann;H. Zahner H.-P. Fiedler;R. Utz;R. Kellner;G. Jung
  16. Appl. Microbiol. Biotechnol. v.30 Fermentation and isolation of epidermin, a lanthionine containing polypeptide antibiotic from Staphylococcus epidermidis Horner, T.;H. Zahner;R. Kellner;G. Jung
  17. Int. J. Food Microbiol. v.42 Characterization of a bacteriocin produced by Streptococcus thermophillus 81 Ivanova, I.;V. Miteva;T. Stefanova;A. Pantey;I. Budakov;S. Danova;P. Moncheva;I. Nikolova;X. Dousset;P. Boyaval
  18. J. Biocteriol. v.167 Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481 Joerger, M.C.;T.R. Klaenhammer
  19. Mammalian Protein Metabolism v.3 Evolution of protein molecules Jukes, T. H.;C. R. Cantor;H. N. Munro(ed.)
  20. J. Mirobiol. Biotechnol. v.8 Bacteriocin produced by Lactobacillus curvatus SE1 isolated from Kimchi Kim, S. K.;E. J. Lee;K. Y. Park;H. K. Jun
  21. Biochimie v.70 Bacteriocins of lactic acid bacteria Klaenhammer, T. R.
  22. Appl. Microbiol. Biotechnol. v.50 Influence of temperature and pH on production of two bacteriocins by Leuconostoc mesenteroides subsp. mesenteroides FR52 during batch fermentation Krier, F.;A. M. Revol-Junells;P. Germain
  23. Appl. Environ. Microbiol. v.57 Partial purification characterization of a bacteriocin produced by Propionibacterium thoenii Lyon, W. J.;B. A, Glatz
  24. Appl. Environ. Microbiol. v.59 Isolation and purification of propionicin PLG-1, bacteriocin produced by a strain of Propionibacterium thoenii Lyon, W. J.;B. A. Glatz
  25. Biochemical Tests for Identification of Medical Bacteria (2nd ed.) Macfaddin, J.F.
  26. Nippon Shokuhin Kogyo Gakkaishi(in Japanese) v.35 Selective media for enumerating lactic acid bacteria groups from fermented pickles Miyao. S.;T. Ogawa
  27. Abstract Book 8th International Biotechnology Symposium v.17 Characterization of the Steptococcus lactis bacteriocin lacticin DPI Piard, J. C.;F. Delorme;M. Desmazeaud
  28. Arch. Microbiol. v.154 Caseicin 80: Purification and characterization of a new bacteriocin from Lactobacillus casei Rammelsberg, M.;E. Muller;F. Radler
  29. J. Gen. Microbiol. v.127 Production, purification and chemical properties of an antistaphylococcal agent produced by Staphylococcus epidermidis Sahl. H. G.;H. Brandis
  30. Mol. Biol. Evol. v.4 The neighbor-joining method:a new method for reconstructing phylogenetic trees Saito, N.;M. Nei
  31. Bergey's Manual of Systematic Bacteriology v.2 Sneath, P. H. A.;N. S. Mair;M. E. Sharpe;J. G. Holt
  32. Curr. Microbiol. v.28 Properties of two bacteriocins synthesized by Leuconostoc strains Sudirman, I.;F. Mathieu;V. Benoit;L. Gerard
  33. Nucleic Acids Res. v.22 CLUSTAL W: Improving the sensivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice Thompson, J. D.;D. G. Higgins;T. J. Gibson
  34. J. Appl. Bacteriol. v.78 Influence of growth conditions on the production of lactonin 705, a bacteriocin produced by Lactobacillus casei CRL 705 Vignolol, G.M.;M.N.de Kairuz;A. A. P.de Ruiz Holgado;G. Oliver
  35. Int. J. Syst. Bacteriol. v.48 Inter-and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences Yoon, J. H.;S. T. Lee;Y. H. Park