Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Facile Purification and Characterization of Dextransucrase from Leuconostoc mesenteroides B-512FMCM

  • Kim, Do-Man (Department of Biochemical Engineering, and the Research Institute for Catalysts, Chonnam National University, Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Kim, Do-Won (Department of Physics, Kangnung National University)
  • Published : 1999.04.01
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Abstract

A simple sequence of membrane concentration and DEAE-Cellulose chromatography has been optimized to give a purified dextransucrase from Leuconostoc mesenteroides B-512FMCM with the highest specific activity (248.8 IU/mg protein) ever reported in high yield (overall 88.7%) for dextransucrase. When there was no sucrose in the dextransucrase and the dextran reaction digest, the dextransucrase hydrolyzed glucose from dextran. The glucose was transferred to the other glucoses from dextran and formed isomaltose and isomaltodextrin. The transglycosylation efficiency of glucose from dextran was much higher with acceptors. The dextransucrase can be used for the production of various kinds (or structures) of oligosaccharides using dextran and various acceptors with almost 100% theoretical yield.

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