Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Characterization of an Acidic Polysaccharide from Fruiting Bodies of Lyophyllum shimeji

  • Lee, Jae-Hoon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Soo-Muk (Korea Research Institute of Bioscience and Biotechnology) ;
  • Han, Sang-Bae (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hwan-Mook (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoo, Ick-Dong (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 1999.04.01
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Abstract

An acidic polysaccharide H-III was extracted from fruiting bodies of Lyophyllum shimeji with hot water. Acid hydrolysis and gas chromatography analysis showed that the polysaccharide was almost exclusively composed of glucose with a very small amount of mannose and galactose. Uronic acid of 8.36% was also detected in H-Ill. Its molecular weight was estimated to be $1{\times} 10^6 Da.\;By\;^{13}C-NMR$ analysis, some repeating units of disaccharide were detected in the polymer H-III. The polysaccharide showed a strong mitogenic activity in a dose-dependent manner.

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