Journal of Microbiology and Biotechnology
- Volume 9 Issue 1
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- Pages.32-38
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- 1999
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Characterization of Biosurfactant from Tsukamurella sp. 26A
- Choi, Kyung-Suk (Department of Microbiology, College of Natural Science, Pusan National University) ;
- Kim, Soon-Han (Department of Microbiology, College of Natural Science, Pusan National University) ;
- Lee, Tae-Ho (Department of Microbiology, College of Natural Science, Pusan National University)
- Published : 1999.02.01
Abstract
A biosurfactant produced by Tsukamurella sp. 26A was purified by procedures including acid precipitation, ethylacetate extraction, and adsorption chromatography. The purified biosurfactant reduced the surface tension of water from 72 mN/m to 30 mN/m at a concentration of 250 mg/l, whereas the minimum interfacial tension against n-hexadecane was lowered to 1.5 mN/m at a concentration of 40 mg/i. The compound stabilized oil-in-water emulsions with a variety of commercial oils and had strong emulsification and stabilization activities when compared to those of commercial emulsifiers and stabilizers. Surface tension was stable over a broad range of pH (2-12) and temperature (
Keywords
- Biosurfactant;
- glycolipid;
- Tsukamurella sp. 26A;
- surface tension;
- interfacial tension;
- bioemulsifier