한국군사과학기술학회지 (Journal of the Korea Institute of Military Science and Technology)
- 제2권2호
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- Pages.140-147
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- 1999
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- 1598-9127(pISSN)
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- 2636-0640(eISSN)
신경작용제 분해효소의 Soman 및 Sarin Gas 분해 능력 측정 및 제독응용 가능성
Test of Degradation of Soman and Sarin Gas by Organophosphorus Acid Anhydrolase and Applicability of the Enzyme to the Development of Nerve Agent Decontaminant
초록
A gene expressing organophosphorus acid (OPA) anhydrolases have been cloned from Alteromonas haloplanktis strain and expressed in bacterial strain BL21. Crude extract was prepared from the transformed bacterial strain BL2l and used in testing its degrading capability of real nerve gas, soman and sarin. Within 1 minute after the start of the reaction, nearly 65% of the soman added to the reactant(3mM) was degraded by adding 1 mg of the crude extract enzyme(20.0 Unit