Characterization of YS-27, an axenic Korean strain of Entamoeba histolytica

  • Park, Soon-Jung (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Yong, Tai-Soon (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Chang, Jae-Kyung (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Im, Kyung-Il (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Soh, Chin-Thack (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine)
  • Published : 1999.03.01

Abstract

Characterization of YS-27, an axenic Entamoeba strain. was performed by three different laboratory methods. Zymodeme analysis using starch gel electrophoresis and PCR with species-specific primers showed that YS-27 is a pathogenic Entamoeba which belongs to the group II zymodeme. Pathogenicity of YS-27 was further confirmed by observing the formation of liver abscess in Mongolian gerbils. These results showed that YS-27 is E. hisolytica.

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