Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Detection of Dirofilaria immitis by Reverse Transcription Polymerase Chain Reaction in Canine

역전사중합효소연쇄반응을 이용한 개심장사상충의 검출

  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to clarify the more accurate detecting method of Dirofilaria immitis. Seven dogs, average 7.47 years old, confirmed with Dirofilaria immitis infection by modified Knott's method were used as the experimental animals. cDNA was constructed using oligodT(15) primer after extracting total RNA from the blood of dogs that were confirmed with Dirofilaria immitis infection. As a result of polymerase chain reaction with template using constructed cDNA, the predicted products of a 378 base-pair DNA fragment was amplified. From these results, RT-PCR was more sensitive and effective than modified Knott's method to detect Dirofilaria immitis in dogs.

Keywords

References

  1. Advanced veterinary parasitology(1st ed.) Rhee,J.K.
  2. J Korean Vet Med Assoc v.21 no.8 진도견의 심장사상충 감염율 조사 김자숙;김선흥;이태욱(등)
  3. J Korean Vet Med Assoc v.28 no.6;Jun 국내 사육견의 심장사상충 실태조사 이상목;최석화;이현하(등)
  4. Korean J Vet Res v.6 no.1 아세톤 집충법에 의한 전주지방축견의 견사상충 감염율 조사 이재구
  5. Observation on the infection rate of helminths in Korean autochthonal dogs with special referense to the viewpoint of public health v.12 Rhee,J.K.;Rim,B.M.
  6. Korean J Parasitology v.36 no.4 Periodicity exhibited by Dirofilaria immitis microfilariae in dogs of Korea Rhee,J.K.;Yang,S.S.;Kim,H.C.
  7. Korean J Parasitology v.34 no.4;Dec A survey of canine heartworm infections German shepherds in south Korea Lee,J.C.;Lee,C.Y.;Shin,S.S.(et al.)
  8. JAVMA v.180 no.1;Jun Four types of occult dirofilaria immitis infection in dogs Rawlings,C.A.;Dawe,D.L.;McCall,J.W.(et al.)
  9. Am J Vet Res v.47 no.2;Feb Canine Dirofilaria immitis infection in a hyperenzootic area : Examination by parasitologic findings at necropsy and by two serodiagnotic methods Grieve,R.B.;Glickman,L.T.;Bater,A.K.(et al.)
  10. Am J Vet Res v.48 no.5;May Comparison of four serotests for the detection of Dirofilaria immitis infection in dogs Thilsted,J.P.;Whorton,J.;Hibbs,C.M.(et al.)
  11. JAVMA v.192 no.10;May Comparison of serologic tests for detection of antigen in canine heartworm infections Brunner,C.J.;Hendrix,C.M.;Blagburn,B.L.(et al.)
  12. Am J Vet Res v.57 no.8;Aug Evaluation of ivermectin and milbemycin oxime efficacy against Dirofilaria immitis infections of three and four months' duration in dogs McCall,J.W.;McTier,T.L.;William,G.R.(et al.)
  13. Mol Biochem Parasitol v.54 no.1;Aug Molecular characterization of a Dirofilaria immitis cDNA encoding a highly immunoreactive antigen Culpepper,J.;Grive,R.B.;Friedman,L.(et al.)
  14. J Med Enomol v.32 no.6;Nov Polymerase chain reaction-based method for the detection of canine heartworm(Filarioidea : Onchocercidae) in mosquitoes(Diptera : Culicidae) and vertebrate hosts Scoles,G.A.;Kambhampati,S.
  15. Tran. Ray. Soc Trop Med Hyg v.33 A method for making microfilaria surveys on day blood Knott,J.