Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Detection of Phagocytosis-Promoting Factor of Culture Supernatant from Feline Peripheral Blood Mononuclear Cells Cultured with Egg White Derivatives

계난백유래물질로 배양한 고양이 말초혈액 단핵구세포 배양상층액중의 탐식촉진인자 검출

  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study is to determine the phagocytosis-promoting factor(s) for feline peripheral blood polymorphonuclear cells (PMN) by culture supernatant from mono-nuclear cells (MNC) treated with egg white derivatives (EWD). The phagocytic activity of PMN was analyzed by a flow cytometry system. The EWD did not show direct effect on the phagocytic response of PMN. The phagocytic activity of PMN was enhanced by culture supernatant from MNC but not PMN treated with EWD. Therefore, it was suggested that the enhanced phagocytic activity of feline PMN could be mediated by humoral factor(s) released from MNC treated with EWD. Thus, the phagocytosis-promoting factor(s) in supernatant fraction from MNC culture treated with EWD were isolated by reverse phase high pressure liquid chromatography. The resulting supernatant fraction on 29.02 minutes of retention time showed high phagocytic activity of PMN. The molecular weight of this supernatant fraction was 16 to 18 kDa when analyzed by capillary electrophoresis. The isoelectric point was pH 5.76 when assessed by ion-exchange chromatography. These results suggest that EWD stimulates feline MNC to elaborate a phagocytosis-promoting factor, 16 to 18 kDa of molecular weight, which could be an important mediator for the enhancement of phagocytic activity of feline peripheral blood phagocytes. Further study will be needed to elucidate this phagocytic factor.

Keywords

References

  1. J Immunol v.147 Differential effects of neutrophil-activating peptide 1/Il-8 and its homologoues on leukocyte adhesion and phagocytosis Detmers,P.A.;Powell,D.E.;Waltz,A.;Clark-Lewis,I.;Baggiolini,M.;Cohn,Z.A.
  2. J Exp Med v.171 Neutrophil-activating protein 1/interleukin 8 stimulating the binding activity to the leukocyte adhesion receptor CD116b/CD18 on human neutrophils Detmers,P.A.;Lo.S.K.;Olsen-Egbert,E.;Waltz,A.;Baggiloini,M.;Cohn,Z.A.
  3. Biochem J v.264 Staurosporine inhibits the respiratory burst and induces exocytosis in human neutrophils Dewald,B;Thelen,M.;Wymann,M.P.;Baggiolini,M.
  4. J Immunol v.114 Functional activation of human neutrophil by recombinant monocyte-derived neutrophil chemotactic factor/IL-8 Djeu,J.Y.;Matsushima,K.;Oppenheim,J.J.;Shiotsuki,K.;Blanchard,D.K.
  5. J Immunol v.139 Down regulation of the receptors for tumor necrosis factor by interleukin 1 and 4β-phorbol-13-acetate Helmut,H.;David,W.
  6. J Molecular Evolution v.41 The tendency of lentiviral open reading frames to become A-rich: constraints imposed by viral genome organization and cellular tRNA availability Hemert,F.J.;Berkhout,B.
  7. Bull Soc Fr Jpn Sci Vet v.6 no.2 Immunostimulating activities of chicken egg white derivaties (EWD) are recombinant bovine interferon alpha 1 (rBoINF α1) in dogs and cattle Hirota,Y.;Yang,M.P.;Araki,S.;Mohaned,A.;Matsumoto,Y.;Onodera,T.;Sugii,S.;Akiyama,K.
  8. J Vet Med Sci v.57 no.5 Enhancing effects of chicken egg white derivatives on the phagocytic response in the dog Hirota,Y.;Yang,M.P.;Araki,S.;Yoshihara,K.;Furusawa,S.;Yasuda,M.;Mohamed,A.;Matsumoto,Y.;Onodera,T.
  9. In vaccines in agriculture: Immunological application to animal health and production Usefulness of egg white derivatives (EWD) and recombinant bovine interferon alpha 1 (rBoINF α1) in veterinary medicine Hirota,Y.;Yang,M.P.;Ohta,Y.;Araki,S.;Matsumoto,Y.;Kang,C.B.;Mohamed,A.;Yoshihara,K.;Furusawa,S.;Suzuki,K.;Onodera,T.;Akiyama,K,;Sugii,S.
  10. J Exp Med v.175 Tumor necrosis factor α directly and indirectly regulates hematopoietic progenitor cell proliferation: Rple of colony-stimulating factor receptor modu-lation Jacobsen,S.W.;Ruscetti,F.W.;Dubois,C.M.;Keller,J.R.
  11. Immunol Lette v.15 Interleukin-2 inhibits migration and stimulates the respiratory burst and degradation of human neutrophils in vitro Kowanko,I.C.;Ferrante,A.
  12. Cytokine v.10 no.7 Purification and characterization of interleukin 1β processing in vitro not involving interleukin 1β convertase Maria,B.;Torbjorn,E.
  13. J Immunol v.138 Tumor necrosis factor is chemotactic for monocytes and polymorphonuclear leukocytes Ming,W.J.;Bersani,L.;Mantovani,A.
  14. J Vet Med Sci v.52 no.2 Effects of active egg white product on neutrophil function in calves Nakagawa,J.;Osame,S.;Ichijyo,S.;Araki,S.;Kimura,M.
  15. Infect Immune v.61 no.8 Interleukin-8 enhances nonoxidative intracellular killing of Mycpbacterium fortuitum by human granulocytes Nibbering,P.H.;Pos,O.;Stevenhagen,A.;Furth,R.V.
  16. Korean J Vet Clin Med v.15 no.1 Effect of ginseng saponins on phagocytosis of feline peripheral blood phagocytes Park,S.E.;Yun,Y.W.;Yang,M.P.
  17. EMBO J v.7 Crystal structure of the cytokine interleukin-1β Priestle,J.P.;Schar,H.P.;Crutter,M.G.
  18. PRoc Natl Acad Sci v.86 Crystallographic refinement of interleukin 1β at 20 Å resolution Priestle,J.P.;Schar,H.P.;Grutter,M.G.
  19. Immunology(3rd ed.) Roitt,I.M.;Brostoff,J.;Male,D.K.
  20. Blood v.83 no.11 Bifunction effects of tumor necrosis factor α (TNF α) on the growth of mature and primitive human hematopoietic progenitor cells: involvement of p55 and p75 TNF receptors Rusten,L.S.;Jacobsen,F.W.;Lesslauer,W.;Loetscher,H.;Smeland,E.B.;Jacobsen,S.W.
  21. Immunology v.82 Macrophageneutrophil interaction: contrasting effects of the monokine interleukin-1 and tumor necrosis factor (chachetin) on human neutrophil adherence Seow,W.K.;Thong,Y.H.;Ferrante,A.
  22. Clin Exp Immunol v.109 Protective effect of granulocyte colony-stimulating factof (G-CSF) in a granulocytopenic mouse model of Pseudomonas aeruginosa lung infection thought enhanced phagocytosis and killing by alveolar macrophages through priming tumor necrosis factor-alpha (TNF-α) production Shirai,R.;Kadota,J.;Tomono,K.
  23. Science v.215 Phagocytosis: Flow cytometric quantitation with fluorescent microspheres Steinkamp,J.A.;Wilson,J.S.;Saunders,G.C.;Stewart,C.C.
  24. The cytokine handbook(2nd ed.) Thomson,A.W.