식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

낙엽송 (Larix leptolepis) 성숙배로부터 부정아 유도 및 식물체 재분화

Adventitious Bud Induction and Plant Regeneration from Mature Embryos in Larix leptolepis

  • 김용욱 (임업연구원 임목육종부) ;
  • 김준철 (강원대학교 생물학과) ;
  • 윤양 (임업연구원 임목육종부) ;
  • 노의래 (임업연구원 임목육종부) ;
  • 손성호 (임업연구원 임목육종부)
  • Kim, Yong-Wook (Department of Tree breeding, Forestry Research Institute) ;
  • Kim, Joon-Chul (Department of Biology, Kangwon National University) ;
  • Youn, Yang (Department of Tree breeding, Forestry Research Institute) ;
  • Noh, Eu-Rae (Department of Tree breeding, Forestry Research Institute) ;
  • Son, Sung-Ho (Department of Tree breeding, Forestry Research Institute)
  • 발행 : 1999.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 낙엽송 (Larix leptolepis)의 종자배로부터 배배양을 통한 식물체 재분화까지의 최적조건을 구명코저 수행되었는데 그 결과는 다음과 같이 요약할 수 있다. 최대 부정아 유도율은 SH배지에 1.0mg/L zeatin을 첨가한 처리구에서 가장 높은 91.7%의 부정아 유도율을 보였으며, cytokinin 혼용처리를 한 부정아 유도는 1.0mg/L zeatin + 1.0mg/L TDZ처리구에서 40.3%로 가장 높은 유도율을 보였다. 그리고 평균 부정아 유도수는 1.0mg/L zeatin+1.0 mg/L 2iP 및 1.0mg/L zeatin+1.0 mg/L kinetin 처리구에서 종자배당 1.9개로 동일한 유도수를 보여 그 효과는 비슷하였다. 부정아의 길이생장은 염류량을 절반으로 줄인 1/2LM배지에서 계대배양하였을 때 가장 높은 27mm의 신초가 생장되어 가장 효과적인 처리구 나타났다. 증식된 신초의 발근율은 5.0 mg/L IBA 및 0.2mg/L NAA 첨가시 23.3%로 가장 높았고 신초에서 유도된 뿌리수는 5.0mg/L IBA처리구에서 신초당 3.5개로 가장 많았다.

Adventitious buds were produced from the cultures of mature zygotic embryos of Larix leptolepis with the highest frequency of 91.7% in SH medium containing 1.0 mg/L zeatin. The most effective cytokinin combination for inducing adventitious buds was 1.0 mg/L zeatin + 1.0 mg/L thidiazuron (40.3%). The highest mean number of adventitious buds induced in 1.0 mg/L zeatin + 1.0 mg/L 2iP or 1.0 mg/L zeatin + 1.0 mg/L kinetin combined treatments. Elongation of the adventitious buds occurred the best on half strength LM salts medium, on which the buds elongated upto 27 mm. Also, the supplement of activated charcoal in medium suppressed the elongation of the adventitious buds. The highest frequency (23.3%) of rooting from elongated shoots was obtained on the medium containing 5.0 mg/L IBA and 0.2 mg/L NAA. The highest number (3.5) of roots was induced on the medium containing 5.0 mg/L IBA alone.

키워드

참고문헌

  1. Plant Cell Tiss Org Cult v.26 Adventitious shoot formation in cultures of 30-year-old Larix decidua, L. leptolepis, L. eurolepis, and L. laricina trees Bonga JM;Pond SE
  2. Plant Sci Lett v.5 Adventitious bud formation in culture of Douglas fir(Pseudotsuga menziesii Mirb) Franco.) Cheng TY
  3. Can For Ser Res Notes v.3 Origin and early development of roots in plantlets derived from dmbryo sections of Larix laricina in vitro Bonga JM;Mclnnis AH
  4. HortSci v.25 In vitro multiple bud formation by 20-year-old western larch buds and atems Chesick EE;Bilderback DE;Blake GM
  5. Plant Cell Tiss Org Cult v.36 Plantlet multiplication from white pine (Pinus strobus L.) embryos in vitro: bud induction and rooting Chesick EE;Mohn CA;Hackett WP
  6. Tappi, Atlanta Potential for genetic engineering in Larix. In : Research and Development Conference Diner AM;Karnosky DF
  7. J For Sci v.16 Initiation, elongation and remultiplication of Larix decidua micropropagules Diner AM;Strickler A;Karnosky D
  8. Plant Cell Tiss Org Cult v.27 A comparison of BAP, zeatin and thidiazuron for adventitious bud formation from Picea glauca embryos and epicotyl explants Ellis DD;Barczynska H;McCown BH;Nelson N
  9. Sil Gen v.42 A system for repeatable formation of elongating adventitious buds in Norway spruce tissue cultures Ewald D;Suss R
  10. New Zealand Appl For v.3 A vegetative propafation system for tamarack Farmer RE;Foster HA;Bakowsky O;MacDonald B;O'keilly G;Reinholt R
  11. Planta v.107 Development and differentiation of haploid Lycopersicon esculentum(tomato) Gresshoff PM;Doy CH
  12. Plant Cell Tiss Org Cult v.41 Plnatlet regeneration from mature embryos of Juniperus cedrus Harry IS;Pulido CM;Thorpe TA
  13. Tress Physiol v.3 In vitro plantlet formation from embryonic explants of eastern white cedar (Thuja occidentalis L) Harry IS;Thompson MR;Lu CY;Thorpe TA
  14. Plant Cell Tiss Org Cult v.37 Regeneration of plantlets through organogenesis from mature embryos of jack pine Harry IS;Thorpe TA
  15. Techniques for increasing clone sizes in arix. In : Proc 6th Int Congr Tissue and Cell Culture Karnosky DF;Diner AM
  16. For Sci v.2 Factors affecting in vitro adventitious bud induction from excised embayos of Swiss stone pine (Ponus embra L.) Lambardi M;Capuana M;Sozzi L;Giannini R
  17. In Vitro Cell Biol v.29 Optimization of in vitro bud induction and plantlet formation from mature embryos of aleppo pine(Pinus halepensis Mill) Lambardi M;Sharma KK;Thorpe TA
  18. Plant Cell Rep v.4 Influence of a loblolly pine(Pinus taeda L.) culture medim and its components on growth and somatic embryogenesis of the wild carrot (Darcus carota L.) Litvay JD;Verma DC;Johnson MA
  19. Bot Gaz v.152 Plantlet regeneration from cultured embryos and seedling parts of red spruce(Picea rubens Sarg.) Lu CY;Harry IS;Thompson MR;Thorpe TA
  20. For Chron v.63 Methods for rooting of larch cuttings and application in clonal selection Morgenstern EK
  21. Acta Horticulture v.78 E'tude de milleux adates aux cultures in vitro de Prunus Quoirin M;Lepoivre P
  22. Tree Physiol v.17 In vitro regeneration of plantlets from mature embryos of Pinus ayacahuite Saborio F;Dvorak WS;Donahue JK;Thorpe TA
  23. Can J Bot v.50 Medium and techniques for induction and growth of monocotyledonous plant cell cultures Schenk RV;Hildebrandt AC
  24. Can J For Res v.24 In vitro micropropagation of Afghan pine Sen S;Magallanes-Cedeno ME;Kamps RH;McKinley CR;Newton RJ
  25. Can J Bot v.68 Pattern of root initiation by an ectomycorrhizal fungus on hypocotyl cuttings of Larix laricina Stein A;Fortin A