Effect of Chemo-and Thermotherapy on LSV elimination in Lilium Oriental Hybrid

나리 LSV 제거를 위한 약제 및 열처리 효과

  • Seo, Sang-Young (Chollabuk-do Agricultural Research and Extension Services) ;
  • An, Min-Sil (Chollabuk-do Agricultural Research and Extension Services) ;
  • Choi, So-Ra (Chollabuk-do Agricultural Research and Extension Services) ;
  • Im, Hoi-Chun (Chollabuk-do Agricultural Research and Extension Services) ;
  • Ryu, Jung (Chollabuk-do Agricultural Research and Extension Services)
  • Published : 1999.01.01

Abstract

Effect of chemo- and thermotherapy on LSV elimination was investigated in Lilium Oriental Hybrid cv. Casa Blanca which was infected by LSV. Either 20 mg/L ribavirin or the treatment of 35$^{\circ}C$/$25^{\circ}C$ (day/night) thermotherapy resulted in the desired growth rate and high percentage (86%, 80% respectively) of LSV elimination. When we combinated those application, the rate of LSV elimination was increased with the time of heat treatment, and was 100% by all heat treatment of 16 weeks. After all of the LSV-free plants transferred into the soil, the number of LSV-free plants was 8 plants out of 12 plants and the efficiency of LSV elimination was best in the combination of 20 mg/L ribavirin and 8 weeks of heat treatment. About 21 % of plants kept LSV-free after transferred all of the LSV-free plants into the soil, the others became LSV-infected again.

LSV 제거 효과를 조사하기 위하여 LSV에 감염된 나리 Casa Blanca를 재료로 기내 배양 중에 화학약제 및 열처리를 단독 또는 병형처리하였다. 단독처리시 ribavirin 20 mg/L과 열처리 35$^{\circ}C$/$25^{\circ}C$ (주간/야간)에서 식물의 생육 및 LSV 제거율(각각 86%, 80%)이 ekfs 처리보다 좋았다. 이 두 처리를 병행하였을 경우, 열처리 기간이 길수록 LSV 제거율은 증가하는 경향이었으며, 열처리 16주의 모든 처리에서 ribavirin 농도와 관계없이 100%의 LSV 제거 효과를 보였다. 토양 정식 후 LSV 무병화 정도는 ribavirin 20 mg/L 농도와 열처리 8주 병행처리에서 12개체 중 8개체가 무병화를 보여 가장 효과적이었으며, 전체 조사 수를 기준으로 했을 때 약 21% 정도의 무병화가 유지되었고, 나머지는 토양의 자연 조건에서 다시 바이러스의 복제, 증식이 되는 것으로 보인다.

Keywords

References

  1. Acta Hort v.164 The regeneration of plants free of LSV and TBV from infected lilium bulb-scale explants in the presence of virazole Blom-Bamhoorm GT;Van Artrijk J
  2. Methods in Enzymology v.118 ELISA Techniques Clark MF;Lister RM;Bar Joseph M
  3. PCR Technology Principles and applications for DNA amplifacction Gelfand DH;Erlich AH(ed.)
  4. Plant Dis v.79 Detection of tomato ringspot virus by polymerase chain reaction Griesbach JA
  5. Plant Dis v.79 Comparison of dot blot, ELISA, and RT-PCR assays for detection of two curumber mosaic virus isolates infecting banana in Hawaii Hu JS;Li HP
  6. Ann Phytopath Soc Jap v.60 Elimination of citrus tatter leaf virus from shoots of potted citrus plants by ribavinin Iwanami T;Ieki H
  7. Kor J Plant Pathol v.12 Detection of lilysymptomless virus using RT-PCR technique Joung YH;Jeon JH;Choi KH;Kim HS
  8. J Kor Soc Hort Sci v.37 Eradication of PVS (Potato Virus S) by themo -and chemotherapy in potato tissue culture Kim HS;Jeon JH;Choi KH
  9. J Kor Soc Hort Sci v.37 Production and detection of virus-free lily plants by shoot tip culture and virazole treatment of bulbils Kim JY;Choi ST
  10. The Research and Extention RDA v.38 Elimination of Odontoglossum ringspot virus from orchid by chemotherapy and themotherapy Kim MS
  11. Potato Res v.16 Heat treatment and meristem culture as a means of freeing potato varieties from viruses X and S McDonald DM
  12. Physiol Plant v.15 A revised medium for rapid growth and bioassay with tobacco cultures Murashige T;Skoog F
  13. PCR protocols A guide to methods and applications Rotbart HA;Innis MA(ed.);Gelfeand DH(ed.);Sninsky JJ(ed.);White TJ(ed.)
  14. Ann Phytopath Soc Jap v.60 Detection of tomato spotted wilt virus S RNA in individual thrips by reverse transcription and polymerase chain reaction Shinya T;Ichiro F
  15. Plant Dis v.64 Pathogen-free plants by meristem-tip culture Slack SA
  16. Ann Phytopath Soc Jap v.60 RT-PCR mediated cloning and sequence analysis of lily symptomless virus Takanmatsu S;Lin BN;Furuta H;Makara K