한국동물위생학회지 (Korean Journal of Veterinary Service)
- 제22권4호
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- Pages.377-383
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- 1999
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- 3022-7372(pISSN)
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- 2287-7630(eISSN)
반추동물에서 과립구의 기능에 대한 연구 1. 소의 순환혈액에서 다형핵백혈구의 신속한 분리
Functional studies of granulocytes in ruminants 1. Rapid separation of polymorphonuclear leucocytes from circulating blood in bovine
초록
Polymorphonuclear (PMN) leucocytes are fundamental importance to the body's defense mechanism and play a major role in the local and systemic reactions to infectious disease. Investigation of the physiological and pathological role of the various leucocyte subtypes in host defence mechanisms is dependent upon the isolation of adequate numbers of viable, pure leucocyte fractions. This report describes the separate frequency of PMN leucocytes both from buffy coat layer and from packed RBC layer when bovine peripheral blood was treated with various anti-coagulants such as acid-citrate-dextrose(ACD), ethyldiaminetetraacetic acid(EDTA), sodium citrate and heparin. The separate frequencies of PMN leucocytes from buffy coat layer was 60.4