Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Injection Media Affecting Expression of Transgene Introduced by Direct in vivo Injection into Olive Flounder (Paralichthys of olivaceus) Muscle

  • Dong Soo Kim (Department of Aquaculture, Pukyong National University) ;
  • Chang Hwa Jeong (Research Center for Ocean Industrial Development, Pukyong National University) ;
  • Young Sun Cho (Research Center for Ocean Industrial Development, Pukyong National University) ;
  • Yoon Kwon Nam (Research Center for Ocean Industrial Development, Pukyong National University)
  • Published : 1999.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The potential utility of injection media (sucrose, PEG, and liposome) was demonstrated for direct gene transfer into olive flounder (Paralichthys olivaceus) muscles. Based on the use of sucrose (final cone. 20%), PEG 8,000 (final cone. 10%) or liposome (twice us of DNA injected), the present injection strategy significantly improved the level of transgene expression as well as persistent duration of expression. The increased amounts of expression in DNA injection with sucrose, PEG, and liposome were as high as from 2.1 to 4.9-folds of conventional TE-based DNA injection. The best result was obtained from injections of liposome-encapsulated DNA in which the expression was detectable at least 32 days after injection when compared to only 8-16 days from TE-based injections.

Keywords

References

  1. Mol. Mar. Biol. Biotechnol. v.5 Gene expression in rainbow trout (Oncorhyncus mykiss) follwing in tramuscular injections of DNA Anderson, E. D.;D. V. Mourich;J. C. Leong
  2. Mol. Mar. Biol. Bioechnol. v.5 Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus Anderson, E. D.;D. V. Mourich;S. C. Fahrenkrug;S. Lapatra;J. Shepherd;J. A.Leong
  3. Circulation Res. v.73 Efficient gene transfer into myocarium by direct injection of adenovirus vectors Guzman, R. J.;Lemarchand, P.;Crystal, R. G.;Epstein, S. E.;Finkel T.
  4. Transgenic Res. v.7 A simple clearing method for improved visualizaiton of lacZ expression in fish larvae Nam, Y. K.;C. H. Jeong;D. S. Kim
  5. Misgurnus mizolepis. Aquaculture v.172 Transmission and expression of an integrated reporter construct up to three generations of transgenic mud loach Nam, Y. K.;C. H. Noh;D. S. Kim
  6. J. Aquacult. v.10 Production of transgenic olive flounder (Paralichthys olivaceus). I. In vivo gene transfer in olive flounder by direct intramuscular injection Nam, Y. K.;S. D.Chu;C. H. Jeong;I. C. Bang;S.-B. Hur;D. S. Kim
  7. Mol. Mar. Biol. Biotechnol. v.1 Fish transgene expression by direct injection into fish muscle Rahman, A.;N. Maclean
  8. Mol. Mar. Biol. Biotechnol. v.6 Efficient gene transfer into zebrafish skeletal muscle by intramuscular injection of plasmid DNA Tan, J. -H.;W. K. Chan
  9. Nature v.389 Gene therapy - promises, problems and prospects Verma, I.M.;N. Somia
  10. Science v.247 Direct gene transfer into mouse muscle in vivo Wolff, J. A.;R. W. Malone;P. Williams;W. Chong;G. Acsadi;A. Jani;P. L. Felgner
  11. Biotechniques v.11 Conditions affecting direct gene transfer into rodent muscle in vivo Wolff, J. A.;P. Williams;G. Acsadi;S. Jiao;A. Jani;W. Chong