Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

Characterization and Immunological Analysis of Insecticyanin from the Hemolymph of Agrius convolvuli

  • Lee, Bo-Young (Department of Biology, College of Sciences, Korea University) ;
  • Lee, Chang-Seok (Department of Biology, College of Sciences, Korea University) ;
  • Lee, Sang-Dae (Department of Biology, College of Natural Sciences, Seonam University) ;
  • Yun, Chi-Young (Department of Biology, College of Sciences, Taejon University) ;
  • Kim, Woo-Kap (Department of Biology College of Sciences, Korea University) ;
  • Kim, Hak-Ryul (Department of Biology College of Sciences, Korea University)
  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A blue biliprotein, insecticyanin (INS), has been purified from the last instar larval hemolymph of Agrius convolvuli by ultracentrifugation, Sephadex G-100 gel permeation chromatography, and preparative electrophoresis. The molecular mass of INS was estimated to be 26 kDa and the N-terminal sequence of INS revealed high similarity to that of Manduca sexta. Results of Western blotting and autoradiography indicated that INS is synthesized by the epidermis and released into the hemolymph. In contrast to the INS reported in other insects, Agrius convolvuli INS contained a small amount of lipid, predominately consisting of triacylglycerol. Subcellular localization of INS was determined using protein-A gold particles linked to secondary antibodies (anti-rabbit Ig). INS was heavily accumulated in the cytoplasmic inclusion body (CIB). CIBs showed a variety of shapes from rod to globule and generally surrounded the nucleus. They were mostly located near the basement membrane and especially abundant in the intersegmental membrane.

Keywords

References

  1. J Histochem Cytochem v.34 Modification of the protein A-Gold immunocytochemical technique for the enhancement of its efficiency Bendayan M;Duhr M
  2. Biochem Biophys Acta v.48 Purification and characterization of a biliverdin-binding cyanoprotein from the locust hemolymph Chino H;Abe U;takahashi K
  3. Insect Biochem v.20 Purification and characterization of biliverdin-associated cyanoprotein from eggs and hemolymph of the bean bug, Ritortus clavatus (Heteroptera:Alydidae) Chinzel Y;Haruna T;Miura K;Numata H;Nakayama S
  4. J Insect Physiol v.15 Cuticular pigments of tobacco hormworm (Manduca sexta)larvae; effects of diet and genetic differences Dahlman D L
  5. Ann N Y Acad Sci v.121 Disc electrophoresis Ⅱ. Methods and application to human serum proteins Davis BJ
  6. J Biol Chem v.226 A Simple method for the isolation and purification of total lipids from animal tissues Folch J;Lees M;Sloane-Stanley GH
  7. Am Zool v.22 purification and characterizationof an epidermal and hemolymph protein from Manduca sexta Goodman WG;Adams B;Carlson RO
  8. Biochemistry v.24 Purification and characterization of a biliverdin-associated protein from the hemolymph of Manduca sexta Goodman W;Adams GB;Trost JT
  9. J Histochem Cytochem v.32 Unlabeled antibody methods in electron microscopy: a comparison of single and multistep procedures using colloidal gold Gosselin EJ;Sorenson GD;Dennett JC;Cate CC
  10. Biochem Biophys Res Commun v.134 A larval specific lipoprotein: purification and characterization of a blue chromoprotein from Heliothis zea Haunerland NH;Bowers WS
  11. Ann Biochem v.161 Purification of very high density lipoproteins by differential density gradient ultracentrifugation Haunerland NH;Ryan RO;Law JH;Bowers WS
  12. Arch Insect Biochem Physiol v.7 Purification and characterization of a very high density chromoprotein from the hemolymph of Trichoplusia ni(Hubner) Jones G;Ryan RO;Haunerland NH;Law JH
  13. Comp Biochem Physiol v.77B Biliverdin Ⅸγ in beetles (Dytiscidae:Laccophilinae) Kayser H;Dettner K
  14. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T₄ Laemmli, UK
  15. Ann Biochem v.74 A new method for determination of molecular weights of proteins by electrophoresis across a sodium dodecyl sulfate (SDS) polyacrylamide gradient gel Lambin P;Rochu D;Fine JM
  16. J Insect Physiol v.13 Pigments in the pupal integuments of two colour types of cabbage white butterfly, Pieris rapae crucivora Ohtaki T;Ohnishi E
  17. Am Zool v.21 Hormonal control of epidermal cell development Riddiford LM
  18. Dev Biol v.92 Changes in translatable mRNAs during the larval-pupal transformation of the epidermis of the tobacco hornworm Riddiford LM
  19. J Biol Chem v.259 The covalent protein structure of insecticyanin, a blue biliprotein from the hemolymph of the tobacco hornworm, Manduca sexta L. Riley CT;Barbeau BK;Kinw PS;Kezd FJ;Heinrikson RL;Law JH
  20. Adv Insect Physiol v.7 The variable coloration of the acridid grasshopper Rowell CHF
  21. Zool Sci v.14 Insecticyanin of Agrius convolvuli, Purification and characterization of biliverdin-binding protein from the larval hemolymph Saito H;Shimoda M
  22. Comp Biochem Physiol v.118B Camparative analysis of storage proteins of the fall webworm (Hyphantria cunea, Drury) Song JK;Nha JH;Kim HR
  23. Ann Ent Soc Am v.64 Extraction and isolation of a blue pigment from third-stage larvae and pupae of the fly Orthillia caesarion (Diptera: Muscidae) Tobin E;Koreeda M;Nakanishi K;Stoffolano JG Jr
  24. Proc Natl Acad Sci USA v.76 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Towbin H;Stahelin T;Gordon J
  25. Insect Biochem v.16 Hemolymph titers of the biliprotein, insecticyanin, during development of Manduca Sexta Trost JT;Goodman WG
  26. Arch Insect Biochem Physiol v.27 Immunological analysis of lipophorin in the haemolymph, ovaries, and testes of the fall Webworm, Hyphantria cunea (Drury) Yun HK;Kim WK;Kim HR
  27. Arch Insect Biochem Physiol v.31 Immunological analysis of apolipophorin-Ⅲ in the haemolymph, ovaries, and testes of the fall Webworm, Hyphantria cunea (Drury) Yun HK;Kim HR
  28. J Cell Biol v.35 Buffered picric-acid formaldehyde; a new rapid fixation for electron microscopy Zamboni L;de Martino C