대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제25권1호
  • /
  • Pages.107-119
  • /
  • 1999
  • /
  • 1226-2587(pISSN)
  • /
  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
권호 표지

Synthesis and Characterization of the Conjugate of Poly(ethylene glycol) with Human Epidermal Growth Factor(hEGF) and its Cell Proliferation Effect

  • Kim, Koi-Ho (R&D Center, Bioland Ltd.) ;
  • Jeong, See-Hwa (R&D Center, Bioland Ltd.) ;
  • Takashi Hirano (Protein Engineering Laboratory, Molecular Biology Department National Institute of Bioscience and Human Technology) ;
  • Park, Soo-Nam (Dept. of Fine Chemistry, Seoul National University of Technology)
  • 발행 : 1999.03.01
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초록

3 Kinds of PEG-hEGF conjugate, PEG5-hEGF, PEG5-hEGF, PEG10-hEGF, were synthesized. Major fractions containing 2 chains of PEG were separated by preparative GPC. Molecular weight was estimated by GPC-MALLS, TOF-Mass and SDS-PAGE, and the data were well corresponding to calculated one. The cell proliferation effect of the conjugates was evaluated, Indicating that the conjugate bound to longer chain PEG exhibits lower activity. Selective modification of hEGF and activity preservation of PEG-hEGF conjugate are undergoing.

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