Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Chemical Properties of the Insecticidal Compound Produced by an Actinomycetes, SS-4993

방선균 SS-4993이 분비하는 살충물질의 화학적 성상 및 작용기작

  • Published : 1999.12.01
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Abstract

The insecticidal compound priduced by Streptomyces SS-4993 strain was indicated to be a relatively stable small compound by pancreatin treatment. The active compound was adsorbed on HP20 and eluted with 70% acetone. Then, the eluate was extracted with ethyl acetate. The content of the extract was determined by thin layer chromatography (Silica gel 60 F254, hexane-ethyl ether-acetic acid,80 : 20 :1). Three active bands (Rf 0.08, 0.07, 0.88) were identified by dermal application using Bombyx mori larvae. Electrophoretic analysis revealed that the injection of strain SS-4993 culture broth into B. mori larvae induce the reduction of the hemolymph proteins level. Especially, the level of apolipophorin-I was decreased drastically.

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References

  1. Modern experimental biochemistry Boyer, R. F.
  2. Archs Biochem. Biophys. v.216 Purification and characterization of chitinase from the stable fly, Stomoxys calcitrams Chen, A. C.;R. T. Mayer;J. R. Deloach
  3. Molecular Biology of the Biological Control of Pests and Diseases of Plants Molecular biology of fungal diseases Christine, D. S.;D. W. Fulbright
  4. The Tools of Biochemistry Cooper, T. G. C.
  5. Approaches to the control of pests and diseases of plants Darrell, J. W.;M. Gunasekaran
  6. gel electrophoresi, Ann. New Nork Acad. Sci. v.121 Disc electrophoresis-Ⅱ. Method and application to human serum proteins Davis, B. J.
  7. J. Antibiotics v.41 A simple screening method for insecticidal substances from actinomyces Fabre, B.;E. Annau;G. Etienne;F. Legendre;G. Tiraby
  8. Agric. Biol. Chem. v.48 The struecture of leucanicidin, a novel insecticidal macrolide produced by Streptomyces halstedii Isogai, A.;S. Sakuda;S. Matsumoto;M. Ogura;K. Furihata;H. Seto;A, Suzuki
  9. J. Antibiotics v.45 AB3217-A, a novel anti-mite substance produced by a strain of streptomyces platensis Kanbe, K.;Y. Mimura;T. Tamamura;S. Yatagai;Y. Sato;A. Takahashi;K. Sato;H. Naganawa;T. Takeuchi;Y. Iitaka
  10. 생물화공 v.6 미생물로부터 생리활성물질의 분리 정제 김창진
  11. Agric. Biol. Chem. v.51 Specific inhibition of Bombyx mori chitinase by allosamidin Koga, D.;A. Iosgai;S. Sakuda;S. Matsumoto;A. Suzuki;S. Kimura
  12. Nature(London) v.227 Cleavage of structural proteins during the assembly of the head bacteriophage T4 Laemmli, U. K.
  13. 한국잠사학회지 v.40 농작물 해충 및 진균류 방제를 위한 방선균의 분리 및 동정 이은정;강경돈;황교열;김두호;김신덕;성수일
  14. J. Antibiotics v.44 Isolation and characterization of new allosamidins Nishimoto, Y.;S. Sakuda;S. Takayama;Y. Yamada
  15. J. Antibiotics v.44 New insecticidal metabilites from soil isolate W719 Reddy, K.;G. Jewett;R. Fatig;M. Brockman;C. Hatton;P. Savickas;D. Hasha;C. Snipes
  16. J. Antibiotics v.40 Search for microbial insect growth regulators allosamidin, a novel insect chitinase inhibitor Sakuda, S.;A. Isogai;S. AMatsumoto;A. Suzuki
  17. J. Antibiotics v.46 Respirantin, a novel insecticidal cyclodepsipetide from streptomyces Urushibata, I.;A. Isogai;S. Matsumoto;A. Suzuki
  18. J. Antibiotics v.46 Biosynthetic studies of allosamidin Zhou, Z. Y.;S. Sakuda;M. Kinoshita;Y. Yamada