한국잠사곤충학회지 (Journal of Sericultural and Entomological Science)

한국잠사학회 (Korean Society of Sericultural Science)
권호 표지

Imporoved Method for the Preparation of Silk Fibroin Hyoysates

  • Shukhrat Madyarov (Institute of physiology and Biophysics, Academy of Sciences of Republic of Uzbekistan, 1 Niyaza6v str., Tashkent-700095, uzbekistan) ;
  • Lee, kawng-Gill (Biomaterial Utiliza6tion Division, Department of Sericulture and Entomology, National Institute of Agricultural Sciences and Technology, Rural Development Adminstration) ;
  • Yeo, Joo-Hong (Biomaterial Utiliza6tion Division, Department of Sericulture and Entomology, National Institute of Agricultural Sciences and Technology, Rural Development Adminstration) ;
  • Jin Nam (Biomaterial Utiliza6tion Division, Department of Sericulture and Entomology, National Institute of Agricultural Sciences and Technology, Rural Development Adminstration) ;
  • Lee, Yong-Woo (Biomaterial Utiliza6tion Division, Department of Sericulture and Entomology, National Institute of Agricultural Sciences and Technology, Rural Development Adminstration)
  • 발행 : 1999.09.01
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초록

An improvement of methods in fibroin hydrolysates preparat significantly enlarges their applications to practical use. Acidic hydrolysis by hydrohloric acid is one of the methods for silk fibroin depolymeriza6tion. A low yield of final product and long time of the process are the demerits of this method. Possibility of preparation of water-soluble silk hydrolysates with more yield and less expenses is investigated in this study. Such possibility is occurred with the increasing tratment temperature and simultaneously decreasing treatment time, concentration of hydrochoric acid respectively, the concentration of sodium hydroxide used for neutraliza6tion of hydolysates after hydrolysis. Colour is decreased in this case and a small amount of activated, too. Protection of hydrolysates against precipitation after neutraliza6tion, and separation and during concentrating process is the other merit of this method. Creamy-coloured insoluble silk powder is the remainder of hydrolyzed fibroin. This is the only the immobiliza6tion of enzymes and other physiological active substances. Fine particles of this powder can be used as additives for artificial diets and cultural media, as well as raw materials for polymer membranes, etc.

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참고문헌

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