Production of the Bacteriocin from the Tofu-Residue

두부비지를 이용한 박테리오신 생산

  • Published : 1999.02.01

Abstract

Growth and bacteriocin production by Lactobacillus sp. GM7311 in tofu residue treated with two commercial amylases were investigated. The optimal condition of amylase Ⅰ(liquefied enzyme for sauce) and Ⅱ(multienzyme 2,000) for the enzyme reaction was showed at pH 6.0 and 4.0, respectively. The optimal temperature was 40oC both. At the enzyme dosage 4% and 3% and reaction time 1hr, about 2% of reduced sugar needed bacteriocin production was obtained. The enzymatic treatment of tofu residue enhanced bacteriocin production by lactic acid bacteria, particularly in the tofu residues added 2.0% yeast extract. But, we couldn't see the increment of bacteriocin activity in the tofu residues added other nitrogen sources such as proteose peptone No. 3 and lab lemco powder. Also, in the comparision of amylase I and Ⅱ, bacteriocin activity in the tofu residue treated with amylase Ⅰ was better than that of amylase Ⅱ.

Keywords

References

  1. J. Nutr. v.80 A comparison of the nutritional value of protein from several soybean fractions Hackler, L. R.;Hand, D. B.;Steinkraus, K. H.;Van Buren, J. P.
  2. Korean J. Food Sci. Technol. v.10 Dehydration of soybean residue by hot-air in conjunction with filter pressing Chung, S. S.;Chang, H. N.;Park, M. Y.
  3. Korean J. Food Sci. Technol. v.17 Some quality changes in soybean curd by addition of dried soymilk residue Sohn, J. W.;Kim, W. J.
  4. Changes in quality of soybean curd residue during drying process and investigation of drying methods, Master Thesis. Sul, M. H.
  5. Korean J. Food Sci. Technol. v.24 Separation of tofu-residue(biji) into dietary fiber and protein fractions Lee, W. J.;Choi, M. R.;Sosulski, F. W.
  6. Kor. J. Appl. Microbiol. Biotech. v.18 Preparation of plant cell wall lytic enzymes from Fusarium moniliforme and protoplast isolation from plant leaf and cell suspension cultures Chung, S. H.;Park, K. H.
  7. Biotechnol. Lett. v.5 Studies on the application of plant cell wall degrading enzymes from Aspergillus torreus and Neurospora crassa Sharma, a.;Joseph, R.
  8. Process Biochemistry v.31 Use of soybean curd residue, Okara, for the solid state substrate in the production of a lipopeptide antibiotic, Iturin a, by Bacillus subtilis NB22 Ohno, A.; Ano, T.;Shoda, M.
  9. Korean J. Food Sci. Technol. v.26 Solubilization of tofu-residue using multienzyme derived from Aspergillus niger CF-34 Kim, K. S.;Park, E. H.;Choi, Y. B.;Kim, K. C.;Lee, S. H.;Sohn, H. S.
  10. Korean J. Food Sci. Technol. v.26 Characterization of tofu-residue hydrolyzing carbohydrase isolated from Aspergillus niger CF-34 Kim, K. S.;Sohn, H. S.
  11. J. Food Prot. v.53 Inhibition of Listeria monocytogenes by bacteriocin producing Pediococcus during the manufacture of fermented simidry sausage Berry, E. D.;Liewin, M. B.;Mandigo, R. W.;Hutkins, R. W.
  12. Inter. J. Food Microbiol. v.52 antimicrobial activity of lactic acid bacteria against Listeria monocytogenes Harris, L. J.;Daeschel, M. A.;Stiles, M. E.;Klaenhammer, T. R.
  13. Appl. Environ. Microbiol. v.58 Nisin dissipates the proton motive forces of the obligate anaerobe Clostridium sporogenes PA 3679 Okereke, A. Thomas, J. M.
  14. Appl. Environ. Microbiol. v.57 Inhibition of food-borne bacterial pathogens by bacteriocin from lactic acid bacteria isolated from meat Lewus, C. B.;Kaiser, A.;Montville, T. J.
  15. Bacteriocins of lactic acid bacteria microbiology, genetics and applications University of Gent, Belgium.
  16. Official methods of analysis. 15th ed. AOAC
  17. Food analysis Ju, H. K.;Cho, H. K.;Cho, H. Y.;Park, C. K.;Cho, K. S.;Chae, S. K.;Ma, S. J.
  18. J. Korean Fish. Soc. v.30 Cultural conditions of Lactobacillys sp. GM7311 for the production of bacteriocin Lee, M. S.;Chang, D. S.;Kang, J. H.
  19. Compendium of methods for the microbiological examination of foods 3rd ed. APHA
  20. Lett. Appl. Microbiol. v.13 Assay system for detecting bacteriocin in microdilution wells Toba, t.;Samant, K. S.;Itoh, T.
  21. Korean J. Food Sci. Technol. v.19 Microbiological studies and biochemical changes in fermenting soybean curd residue during fermentation Lee, M. S.;Kim, K. H.;Lee, G. J.
  22. J. Food Sci. v.48 Application of enzyme in soy milk production to improved yield Eriksen, S.
  23. Korean J. Food Sci. Technol. v.24 Effects of functional properties of soy protein isolate and qualities of soybean curd upon proteolytic hydrolytic hydroysis Han, J. S.;Hwang, I. K.
  24. Korean J. Food Sci. Technol. v.25 modification fo functional properties of soy protein isolate by proteolytic enzymes Cha, M. H.;Yoon, S.
  25. Appl. Microbiol. v.24 Effect of the medium on bacteriocin production among strains of Streptococcus mutans Rogers, A. H.