리베틴에 존재하는 난황항제의 소화효소에 대한 저항성

Resistance of Hen항s Egg Yolk Immunoglobulins in Livetin to Digestive Enzymes

  • 이경애 (순천향대학교 식품영양학과)
  • 발행 : 1999.04.01

초록

A livetin solution(LS) containing yolk immunoglobulins(IgY) was separated by treating the egg yolk with natural gum, carrageenan. Carrageenan has been used as a food ingredient. Relative absorbance of IgY LS after proteolysis was investigated. IgY LS was fairly stable to pepsin digestion at pH 3.0. However, IgY LS appeared to be susceptible to pepsin digestion at pH 2.0, showing 18% of relative absorbance and complete breakdown H chain after 30 min exposure. Relative absorbance of IgY LS was considerably high after exposure to trypsin and chymotrypsin for 8 hr. IgY LS showed especially good stability to chymotrypsin digestion.

키워드

참고문헌

  1. Eur. J. Immunol. v.4 Immunoglobulin classes in the hen's egg : their segregation in yolk and white Rose, M. E.;Orlands, E.;Buttress, N.
  2. J. Virol. Methods v.1 Hen egg yolk as a source of anti-viral antibodies in the enzyme-linked immunosorbent assay(ELISA): a comparison of two plant virus Bar-Joseph, M.;Malkinson, M.
  3. Dev. Com. Immunol. v.5 Immunoglobulins in the egg, embryo and young chick Rose, M. E.;Orlands, E.
  4. J. Vet. Med. B v.33 The chicken egg, an antibody source Losch, U.;Schranner, I.;Wanke, R.;Jurgens, L.
  5. Vet. Bull. v.45 Physical and chemical properties of fowl immunoglobulins Higgins, D. A.
  6. Immunoglobulin characteristics: chicken, in inbred and genetically defined strains of laboratory animals ; Bilolgical handbooks Ⅲ Benedict, A. A.
  7. Cell v.59 Somatic hyperconversion diversifies the single VH gene of the chicken with a high incidence in the D region Reynaud, C-A.;Dahan, A.;Anquez, V.;Weill, J-C.
  8. EMBO J. v.7 Chicken immunoglobulin G-heavy chains: limited VH gene repertoire, combinational diversification by K gene segments and evolution of the heavy chain locus Parvari, R.;Avivi, A.;Lentner, F.;Ziv. E.;Tel-Or, S.;Brustein, Y.;Schechter, I.
  9. Cell v.48 Somatic diversification of the chicken immunohlobulins light chain gene in limited to the rearranged variable gene segment Thompson, C. B.;Meiman, P. E.
  10. J. Exp. Med. v.130 Phylogeny of immunoglobulin structrue and function : Immunoglobulin of the chicken Leslie, G. A.;Clem, L. W.
  11. Can. J. Biochem. Phy. v.35 Fractionation of livetin and other molecular weights of the α-and β-components Martin, G. W.;Vandergaer, J. E.;Cook, W. H.
  12. J. Food Sci. v.53 Separation of phospholipids from egg yolk and recorery of water-soluble proteins Hatta, H.;Sim, J. S.;Nakai, S.
  13. Pediatrics v.81 Antibodies to rotaviruses in chicken's egg: A potential source of anti-viral immunoglobulins suitable for human consumption Yolken, R. H.;Leister, F.;Wee, S-B.;Miscuff, R.;Vonderfecht, S.
  14. Microbiol. Immunol. v.34 Gastroenteritis in suckling mice caused by human rotavirus con be prevented with egg yolk immunoglobulin and treated with a protein-bound polysaccharide perparation Ebina, T.;Tsukada, K.;Umezu, K.;Nose, M.;Tsuda, K.;Hatta, H.;Kim, M.;Yamamoto, T.
  15. Dent. Res. v.70 Protection of rats against dental caries by passive immunization with hen-egg yolk antibodies Otake, S.;Nishihara, Y.;Makimura, M.;Hatta, H.;Kim, M.;Yamamoto, T.
  16. Infect. Immun. v.59 Oral passive immunization against dental caries in rats by use of hen egg yolk anti bodies specific for cell-associated glucosyltransferase of streptococcus mutans Hamada, S.;Horikoshi, T.;Minami, T.;Kawabata, S.;Hiraoka, J.;Fujiwara, T.;Ooshima, t.
  17. Infect. Immun. v.60 Passive pretection of chicken egg yolk immunohlobulins against experimental enterotoxicogenic Escherishia coli infection in neonatal piglets Yokoyama, H.;Peralta, C.;Diaz, R.;Sendo, S.;Ikemori, Y.;Kodama, Y.
  18. J. Korean Soc. Food. Sci. v.12 Studies on the stability of hen's egg yolk immunoglobulins Lee, K. A.
  19. J. Korean Soc. Food. Sci. v.13 Changes of antigen binding activities of hen's egg yolk immunoglobulins after proteolysis Lee, K. A.
  20. E. Coli, N. Engl. J. Med. v.318 Protiction by milk immunoglobulin concentrate against oral challange with enterotoxicogenic Tacket, C. O.;Losonsky, G.;Link, H.;Hoang, Y.;Guesry, P.;Hilpert, H.;Levine, M. M.
  21. Can. J. Anim. Sci. v.57 Survival of colostrum-deprived neonatal piglets fed gamma-globulin McCallum, I. M.;Elliot, J. I.;Owen, B. D.
  22. Immunol. Commun. v.9 Isolation of viral IgY antibodies from yolks of immunezed hems Polson, A.;Von Wetchmar, B.;Fazakerly, G.
  23. Agri. Biol. Chem. v.54 A novel mothod for hen eggyolk antibody, "IgY" Hatta, H.;Kim, M.;Yamamoto, T.
  24. ELISA. First international symposium on immunoenzymatic technique INSERIM symposium No. 2. Enzyme-linked immunosorbent assay Engvall, E.;Perlmann, P.
  25. J. Biol. Chem. v.244 The reliability ofn molecular weight determined by dodecyl sulfate polycrylamide gel electrophoresis Weber, K.;Osborn, M.
  26. Proc. Natl. Acad. Sci. USA v.50 Dissociation of rabbit gamma globulin into subunits by reduction and acidification Palmer, J. L.;Nisonoff, A.;Holde, K. E. V.
  27. Proc. Natl. Acad. Sci. USA v.54 Reductive dissociation of chicken γG immunoglobulin in neutral solvents without a dispersing agent Dressman, G. R.;Benedict, A. A.
  28. J. Immunol. v.104 Strutural and antigenic relationship between abian immunoglobulinsⅡ. Properties of papain-and pepsin-digested chicike, pheasant and quail IgG-immunoglobulins Leslie, G. A.;Benedict, A. A.