Optimization of Extraction Conditions for Soluble Ginseng Components Using Microwave Extraction System under Pressure

가압형 마이크로웨이브 추출장치를 이용한 가용성 인삼성분의 추출조건 최적화

  • Published : 1999.04.01

Abstract

Microwave extraction system equipped with closed vessels was applied to confirm its capability of extracting ginseng solubles including saponin, which was monitored to optimize extraction con ditions by response surface methodology. Total yield increased with the decrease in particles size and more extraction steps. Soluble components were completely extracted by operating the system within 6 min per one step, which should be repeated by 3 or 4 times. Optimized conditions for maxi mum extraction of response variables(total yield, crude saponin) were 40~50% of ethanol concentration, about 140oC of extraction temperature, and within 6 min of the extraction time. The extraction effi ciency of total yield was dependent on the decrease of ethanol concentration, while crude saponin content was favored on the higher degree of ethanol concentration. According to the temperature guideline, below 90oC of extraction temperature, the optimal ranges of extraction conditions were predicted as 30~50% of ethanol concentration and 2.5~6.5 min of extraction time. Estimated values of total yield and crude saponin were in good agreement with experimental values.

Keywords

References

  1. Understanding of Korean Ginseng SKG
  2. Proc. 1st Intl. Ginseng Symp. Pharmacological ctudies in ginseng Takagi, K.
  3. Proc. 2nd Intl. Ginseng Symp. Pharmacological properties of Panax ginseng root Saito, H.;Lee, Y. M.
  4. Chem. Pharm. Bull. v.24 Effect of ginsenh saponins on nuclear ribonucleic acid(RNA) metabolism.Ⅰ. RNA synthesis in rats treated with ginsenosides Iivim, M.;Higashi, T.;Sanada, S.;Shoji, T.
  5. Proc. 1st Intl. Ginseng Symp. Metabolic acrions of ginseng princi;oes in bone marrow and testes Yammamoto, M.;Kumagai, A.;Yamamura, Y.
  6. Arzneim. Forsh. v.27 Stimulatory effect of Panax ginseng principles on DNA, RNA, protein and lipid synthesis in hat bone marrow Yammamoto, M.;Takeuchi, N.;Kumagai, A.;Yamamura, Y.
  7. Proc. 3rd Intl. Ginseng Symp. Endocrinological and metabolic action of ginseng principles Yammamoto, M.;Uemura, T.
  8. Wakan Yaku Gakkaissi v.1 Improving effects of ginseonside-Rb₂in streptozotocin diabetic rat with hyperglycemia and hyperlipermia Yaokazawa, T.;Kobayashi, T.;Oura, H.;Kawashima, Y.
  9. J. Pharm. Dyn. v.5 Effect of a hypoglycerin components on ginseng radix on insulin biosynthesis in normal and diabetic animals Waki, I.;Kyo, H.;Yasuda, M.;Kimura, M.
  10. Japan, J. Pharmacol. v.24 Effect of panax ginseng root on exhausive exercise in mice Saito, H.;Yoshida, Y.;Takagi, K.
  11. Proc. 3rd Intl. Ginseng symp. Adrenocorticotropin and corticost crone secetion by ginseng saponin Hia, S.;Yokoyama, H.;Oura, H.
  12. Korean J. Ginseng Sci. v.18 Optivization of saponin extraction conditions in ginseng milk using response surface methodology Lee, S. S.;Park, J. M.;Oh, H. I.;Kwak, H. S.
  13. Korean Food Standard Code KMHW
  14. J. AOAC Intl. v.79 Microwave-assisted extraction as an alternative to Soxhlet, sonication, and supercritical fluid extraction Lopez-Avila, V.;Young, R.;Teplitsky, N.
  15. Analytical Methods of Ginseng Components KGTRI
  16. Syoyakugaku Zasshi v.25 Chemical studies on the Oriental plant drugs(XXV.). Comparative studies on the saponins and sapogenins of Ginseng and related Crude Drugs Ando, T.;Tanaka, O.;Shibata, S.
  17. Kor. J. Pharmacogn. v.23 Extract yields and saponin contents of red ginseng extracts prepared with various concentrations of ethanol (in Korean) Ko, S. R.;Kim, S. C.;Choi, K. J.
  18. Comprative study on chemical components and biological activities of Panax species. Ph. K. Thesis Ko, S. R.
  19. Response surface methodology Myers, R. H.
  20. J. Food Sci. v.57 Edible wheat gluten films: Influence of the main process variables on film properties using response surface methodology Gontard, N.;Guilbert, S.;Cuq, J. L.
  21. User's Guide VErsion 6. 4th ed. v.2 SAS;SAS/STAT
  22. The Mathematica Handbook, compatible with Mathematica Version 2.0 Martha, L. A.;James, P. B.
  23. Korean J. Food Sci. Technol. v.30 Optimization of extraction conditions for ethanol extracts from Chrysanthemum morifolium by response surface methodology Park, N. Y.;Kwon, J. H.;KIm, H. K.
  24. Studies on the effect of extractiong conditions on the physicochemical properties of Korean ginseng extrct. Ph.D. Dissertation Sung, H. S.
  25. Presented at IFT Annual Meeting, Atlanta, Georgia, USA Optimization of microwave-assisted extraction for effective components of Panax ginseng C. A. Meyer. Kwon, J. H.;Lee, S. B.;Lee, G. D.;Kim, H. K.
  26. Trends in Analytical Chemistry v.13 Microwave-assisted process: a new tool for the analytical laboratory Pare, J. R. J.;Belanger, J. M. R.;Stafford, M. R.
  27. J. Ginseng Sci. v.3 Studies on the extracting methods of ginseng extract and saponins in Panax ginseng(in Korean) Joo, H. K.;Cho, K. S.
  28. Proc. 2nd. Intl. Ginseng Symp. Effect of ginseng saponin on protein synthesis in heart muscle Lee, K. S.