Inhibitory Effect of Coprinus comatus Ethanol Extract on the Liver damage in Benzo(a)pyrene-treated Mice

먹물버섯 에탄올추출물이 Benzo(a) pyrene 투여에 의한 마우스의 간 손상에 미치는 영향

  • 이갑랑 (영남대학교 식품영양학과) ;
  • 이병훈 (영남대학교 식품영양학과) ;
  • 김현정 (영남대학교 식품영양학과) ;
  • 장종선 (영남대학교 식품영양학과) ;
  • 배준태 (영남대학교 식품영양학과) ;
  • 박선희 (영남대학교 식품영양학과) ;
  • 이승언 (영남대학교 식품영양학과) ;
  • 김옥미 (영남대학교 식품영양학과) ;
  • 이별나 (대구공업대학 식품영양과)
  • Published : 1999.12.01

Abstract

This study was carried out to investigate the inhibiton effects of Coprinus comatus ethanol extract of edible mushroom on liver damage in benzo(a)pyrene (B(a)P) treated mice. The activities of serum aminotransferase, cytochrome P 450 and hepatic content of lipid peroxide after B(a)P treatment were increased than those of control, but those levels were significantly decreased by the treatment of Coprinus comatus ethanol extract. Whereas, the hepatic glutathione content and glutathione S transferase activity were decreased by B(a)P treatment than those of control, but those were increased by the treatment of Coprinus comatus ethanol extract. Also the activities of superoxide dismutase, catalase and glutathione peroxidase after B(a)P treatment were markedly increased than those of control, but those levels were decreased by the treatment of Coprinus comatus ethanol extract. These results suggest that Coprinus comatus ethanol extract have a protective effect on liver damage by benzo(a)pyrene through the mechanisms of decreasing lipid peroxide and activities of free radical generating enzymes.

Keywords

References

  1. Korean Mycol. v.20 Medicinal fungi in Korea Ahn,D.K.
  2. J. Korean Soc. Food Sci. Nutr. v.28 Antimutagenic effects of the fruiting body and the mycelia extracts of Coprinus comatus Kim,H.J.;Lee,B.H.;Kim,O.K.;Bae,J.T.;Park,S.H.;Park,D.C.;Lee,K.P.
  3. Physiological Reviews v.60 Benzo(a)pyrene metabolism, activation and carcinogenesis : Role and regulation of mixed-function oxidases and related enzymes Gelbin,H.V.
  4. Cancer Res. v.54 Screening of potential chempreventive agents using biochemical markers of carcinogenesis Sharma,S.;Stutzman,J.D.;Kelloff,G.J.;Steele,V.E.
  5. Toxicology Letters v.82 Chemoprotection against cancer by phase Ⅰ enzyme induction Talalay,P.;Fahey,J.W.;Holtzdaw,D.;Prestera,T.;Zhang,Y.
  6. Anal. Biochem v.95 Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction Ohkawa,H.;Ohishi,N.;Yaki,K.
  7. Arch Biochem. Biophy, v.82 Tissue sulfhydryl group Ellman,G.L.
  8. Am. J. Clin. Pathol. v.28 A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminase Reitman,S.;Frankel,S.
  9. Eur. J. Biochem. v.47 Involvement of the sueroxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase Marklund,S.;Marklund,C.T.
  10. In Methods of enzymatic analysis v.2 Catalse Aebi,H.;Vergmeyer,H.U.(ed.)
  11. J. Lab. Clin, Med. v.70 Studies on the quantitative and qualtiative characterization of erythrocyte glutathione peroxidase Paglia,E.D.;Valentine,W.N.
  12. Anal. Biochem. v.249 Glutathione S-transferase: The first enzymatic step in mercapturic acid reaction Habig,W.H.;Pabst,M.J.;Jakoby,W.B.
  13. J. Biol. Chem. v.239 The carbon monooxide binding pigment of liver microsomes. 1. Evidence for its hemoprotein nature Omura,T.;Sato,R.
  14. J. Biol. Chem. v.193 Protein measurement with folin phenol regent Lowry,O.H.;Rosebrough,N.H.;Farr,A.L.;Randall,R.L.
  15. Science v.120 Transaminase activity in human blood LaDue,J.S.;Wroblewski,F.;Karmen,A.
  16. J. Korean Soc. Food Nutr. v.22 The effect of lithosperimi radix on benzo(a)pyreneinduced hepatotoxicity Yoon,S.H.;Park,E.J.;Oh,K.H.;Chung,Y.G.;Kwon,O.J.
  17. J. Korean Soc. Food Nutr. v.27 Inhibition effects of Auricularia auricular-judae in benzo(a)pyren-treated mice Chang,J.S.;Kim,H.J.;Bae,J.T.;Park,S.H.;Lee,S.E.;Kim,O.M.;Lee,K.R.
  18. In Review in biochemical toxicology Lipid peroxidation and its role in toxicology Bus,I.S.;Gibsin,J.E.
  19. Annu. Rev. Biochem. v.52 Glutathione Meister,A.;Anderson,M.E.
  20. Fed. Proc. v.32 Free radical lipid peroxidation damage and its inhibition by vit E and selenium Tappel,A.L.
  21. A. Biochem. Med. Metabolic Biol. v.44 Modulation of masheri inducible and benzo(a)pyrene inducible carcinogen-metabolizing enzymes by dietary vitamin Ammigan,N.;Nair,U.J.;Bhide,S.V.
  22. In Methods in enzymology Roles of free radicals and catalytic metal ions in human disease Halliwell,B.;Gutterridge,J.M.C.;Fleischer,S.(ed.);Packer,L.(ed.)
  23. Food toxicology, A perspective on the relative risks Steven,L.T.;Richard,A.S.
  24. Europen J. Cell Biology v.22 Control of the biosynthesis of mitochondrial manaanese superoxide-dis-mutase and catalase by oxygen radicals Autor,A.P.;Stevens,J.B.
  25. In Annual review of biochemicsty v.64 Superoxide radical and sueroxide dismutase Friodovich,I.
  26. Nutr. Res. v.38 Structure, synthesis and function of glutathione peroxidase Sunde,R.A.;Hoekstra,W.G.
  27. In Glutathione (3rd ed.) Pysiological activity of glutathione Sakamoto,Y.;Kinoshita,S.