Preventive Nutrition and Food Science
- Volume 4 Issue 1
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- Pages.47-51
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- 1999
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- 2287-1098(pISSN)
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- 2287-8602(eISSN)
Hepatic and Renal cysteine Sulfinic Acid Decarboxylase Activities in Cats Fed Different Levels of Dietary Protein and Taurine
- Park, Taesun (Department of Food and Nutrition Yonsei University) ;
- Quinton R. Rogers (Department of Molecular Biosciences, University of California)
- Published : 1999.03.01
Abstract
In order to evaluate the dietary regulation of cysteine sulfinic acid decarboxylase (EC 4.1.1.29) in cats, acitivity and protein content of CSAD were assessed in the liver and kidney of cats fed different levels of dietary protein, with and without taurine. Four groups of cats were fed one of the follow diets for 5 weeks ; 20% protein and taurine- free diet(LP0T) ; 20% protein and 0.15% taurine diet(LPNT) ; 60% protein and taurine-free diet(HP0T); and 60% protein and 0.15% taurine diet (HPNT). CSAD activity was determined in the liver and kidney of cats by measuring 14C2 released form [1-14C]-L cysteine sulfinic acid. CSAD protein was quantified using an immunochemical method. CSAD activity was extremely low in cat tissues, among which kidney showed the highest activity which was 0.118