The Plant Pathology Journal
- 제15권6호
- /
- Pages.335-339
- /
- 1999
- /
- 2093-9280(eISSN)
Detection of Citrus Tristeza Virus by RT-PCR and Status of CTV Infection among Citrus Trees in Cheju Island
- Oh, Hyun-Jeong (Applied Radioisotope Research Institute, Cheju National University) ;
- Park, Sung-Hugh (Graduate School of Biotechnology, Korea University) ;
- Lee, Se-Yong (Graduate School of Biotechnology, Korea University) ;
- Jeon, Gyeong-Lyong (Faculty of Horticultural and Life Science, Cheju national University) ;
-
Riu, Key-Zung
(Faculty of Horticultural and Life Science, Cheju national University) ;
- U, Zanh-Kual (Faculty of Horticultural and Life Science, Cheju national University)
- 발행 : 1999.12.01
초록
Citrus tristeza virus(CTV), an aphid-borne closterovirus, is one of the most destructive pathogens of citrus. It has caused rapid decline in growth, stem pitting and death in citrus trees. A reverse transcriptase polymerase chain reaction (RT-PCR) assay was developed for detection of CTV and investigation of the CTV infection status of citrus and its related cultivars in Cheju island. For RT-PCR based CTV detection, primers were designed to amplify 670bp of coat protein gene. A screening test for CTV in citrus cultivars was conducted from March to July in 1999. Seventy individual citrus trees representing 9 species of 3 genera were tested. The infection rates of CTV for leaves from the years or older trees of late maturing citrus varieties such as Yuzu (C. junos Sieb. ex Tanaka), Navel orange (C.sinensis Osbeck), Kiyomitanger (C. unshiu x C. sinensis), and Shiranuhi ((C. unshiu x C. sinensis) x C. reticulata) were 100%, 80%, 60%, and 60% respectively. The CTV infection rates in Early satsuma mandarins such as 'Miyagawa Early' Satsuma mandarins (C. unshiu Marc. var. Miyagawa) and 'Okitsu Early' Satsuma mandarins (C. unshiu Marc. var. Okitsu) were 100%, and 60%, respectively. CTV was not detected in Cheju native Dangyooja (C. unshiu Marc. var. Osbeck), Trifoliate orange (Poncirus trifoliata) and Kumquat (Fortunella margarita Swingle). In conclusion, RT-PCR assay can be successfully applied to the detection of CTV in citrus trees.