The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Cloning of $\beta$-Tubulin Gene and Effect of Pencycuron on Tubulin Assembly in Rhizoctonia solani

  • Roh, Seong-Hwan (Department of Agricultural Biology, College of Natural Resources and Life Science, Dong-A University, Pusan) ;
  • Heung-Tae KIm (Pesticide Screening Division, Korea Research Institute of Chemical Technology, Taejon) ;
  • Isamu-Yamaguchi (The Institute of Physical and Chemical Research (RIKEN), Hirosawa, Wako, Japan)
  • Published : 1999.02.01
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Abstract

To illustrate the action mechanism of pencycuron on Rhizoctonia solani, two experiments were conducted including the comparison of amino acids of $\beta$-tubulin between R-C (sensitive isolate) and Rh-131 (non-sensitive isolate), and the inhibitory effect of pencycuron on tubulin assembly in vitro. Both $\beta$-tubulin genes of R-C and Rh-131 proved to have 1,582 nucleotides encoding a protein of 445 amino acids, showing 98% homology in amino acid sequences between them. It was found that codons at 103, 236, and 267 for lysine (AGG), valine (GTC) and isoleucine (ATT) in R-C were replaced by codons for methionine (ATG), isoleucine (ATT) and methionine (ATG) in Rh-131, respectively. No inhibitory effect of pencycuron on the tubulin assembly was observed. It suggests that pencycuron may have no direct inhibitory effects on the assembly of tubulin at least in vitro.

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