Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Follicle Size and Oocyte Type on In Vitro Maturation of Porcine Follicular Oocytes

난포의 크기 및 난포란의 형태가 돼지 난포란의 체외성숙에 미치는 영향

  • 박병권 (공주문화대학 애완동물과) ;
  • 박영석 (공주문화대학 애완동물과) ;
  • 이미영 (공주문화대학 애완동물과) ;
  • 이성호 (공주문화대학 애완동물과) ;
  • 김덕환 (충남대학교 수의과대학) ;
  • 이종완 (공주대학교 산업과학대학) ;
  • 권건오 (공주문화대학 애완동물과) ;
  • 김인봉 (공주문화대학 애완동물과) ;
  • 김형태 (대주산업)
  • Published : 1999.12.01
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Abstract

This study was conducted to find out the effect of follicle size and oocyte type on in vitro maturation of poricine follicular oocytes. TCM-HEPEAS medium was used to basic medium, and the oocyte matured in vitro was stained with the Rapid staining method. The results obtained were summarized as follows; 1. The number of follicles an ovary was 20.5. The number of A-and B-typed oocytes an ovary was 2.34. The proportion of A-and b-types oocytes was 40% of the recovery oocytes. 2. Cumulus expanison indexes(CEI) by the follicle size were 1.62∼2.34(<2mm), 1.27∼2.28(2∼5mm) and 1.46∼2.75(>5mm). It was no differ to maturation rate by the follicle size. 3. The degree of oocyte maturation based on oocyte type did not differ for B-and C-typed oocyted but the index of oocyte type A was higher than that of b-and C-typed oocytes. 4. When follicluar oocytes were cultured for 42 hours, the proportion of the Met-II(second metaphase) stage were 22.5% (degree 1), 35.4%(degree 2) and 65.5% (degree 3).

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