한국수정란이식학회지 (Journal of Embryo Transfer)
- 제14권2호
- /
- Pages.107-111
- /
- 1999
- /
- 2508-755X(pISSN)
- /
- 2288-0178(eISSN)
Bovine Fetal Fibroblasts를 이용한 핵이식 및 세포융합에 관한 연구
Production of Cloned Embryos by Nuclei Transfer and Electronic Cell Fusion from Bovine Fetal Fibroblasts
초록
The present study was performed to evaluate the best electric fusion condition in nuclear transfer, Korean Native Cattle fibroblasts were used as nucleic donors. Oocytes from slaughterhouse were matured in vitro for 22 h and enucleated. Each individual cells were transferred into enucleated ocytes and reconstructed embryo were placed into the fusion chamber. In experiment 1, pulse were performed by altering pulse duration at 1. 75kv/cm, 1 time. When pulse duration is 30