KSBB Journal

  • 제14권3호
  • /
  • Pages.351-357
  • /
  • 1999
  • /
  • 1225-7117(pISSN)
  • /
  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

한천분해효소의 고생산성 변이주의 분리 및 최적배양조건

Isolation and Its Optimal Culture Condition for High Agarase-Producing Mutant

  • 황선희 (부경대학교 식품생명공학부) ;
  • 하순득 (부경대학교 식품생명공학부) ;
  • 김봉조 (부경대학교 식품생명공학부) ;
  • 김학주 (부경대학교 식품생명공학부) ;
  • 공재열 (부경대학교 식품생명공학부)
  • 발행 : 1999.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A marine bacterium Bacillus cereus ASK202, agarase producing strain, was treated with some mutagenic agents, ultraviolte(UV), 1-methyl-3-nitro-1-nitrosoguanidine(NTG), and ethyl methane sulfonate(EMS), several times for the increasing of the agarase production After mutagen treatment, we isolated one mutant strain treated with NTG showed the highest stability and agarase productivity and named as Bacillus cereus ASK202-N3. This Bacillus cereus ASK202-N3 strain was well grown in the modified marine medium containing 0.5%(w/v) agar, 0.3%(w/v) yeast extract, and 5.0%(w/v) NaCl, and the optimal initial pH, temperature and culture time were 7.8, $25^{\circ}C$ and 32h, respectively. In the optimal culture conditions, the agarase production was increased to 5.3 fold(850units/L) compared to that of the wild type.

키워드

참고문헌

  1. 實踐 バイリアクタ-(初版) 食品産業バイリアクタ-ツステム技術硏究組合
  2. Nippon Nogeikagaku Kaishi v.63 no.6 ネオアガロオリゴ糖の特性とその生産技術 河野敏明;日高秀昌
  3. 日本水産學會大會講演要旨集 寒天分解酵素生産菌の分李とその酵素の性質 河野敏明;山口吾一;日高秀昌;三好照三;北川廣進;平賀哲男
  4. Appl. Environ. Microbiol. v.59 no.5 Purfication and Characterization of a New Agarase from a Marine Bacterium, Vibrio sp. Strain JT0107 Sugano, Y.;I. Terada;M. Arita;M. Noma;T. Matsumoto
  5. Agric. Biol. Chem. v.55 no.10 Purfication and Some Properties of Agarase from Pseudomonas sp. PT-5 Yamaura, L.;T. Matsumoto;M. Funatsu;H. Shigeiri;T. Shibata
  6. Biotechnol. Lett. v.19 no.1 Cloning and Expression of an Agarase Gene from a Marine Bacterium Pseduomonas sp. W7 Kong, J. Y.;S. H. Hwang;B. J. Kim;S. K. Bae;J. D. Kim
  7. Eur. J. Biochem. v.135 β-Agarase Ⅰ and Ⅱ from Pseudomonas atlantica - Purification and some Properties Morrice, L. M.;M. W. Mclean;F. B. Williamson;W. F. Long
  8. Appl. Environ. Microbiol. v.54 no.1 Cloning and Gene Replacement Mutagenesis of a Pseudomonas atlantica Agarase Gene Belas, R.;D. Barlett;J. Silverman
  9. Nippon Suisan Gakkaishi v.56 no.5 Purification and Characterization β-Agarase from Vibrio sp. AP-2 Aoki, T.;T. Araki
  10. Mol. Gen. Genet. v.209 tghe Agarase Gene(dag A) of Streptomyces coelicolor A3(2) : Nucleotide Sequence and Transcriptional Anlaysis Buttner, M. J;I. M. Fearnley;M. J. Bibb
  11. Appl. Environ. Microbiol. v.58 no.12 Purification and Properties of an Extracellular Agarase from Alteromonas sp. Strain C-1 Leon, O.;L. Quintana;G. Peruzzo;J. C. Slebe
  12. Essentials of Molecular Biology David Freifelder
  13. 미생물유전학실험 주현규
  14. Korean J. Biotechnol. Bioeng. v.12 no.2 Isolation and Iedntification of Marine Bacterium Bacillus cereus ASK202 and Optimal Culutre Conditions for Production of Agarase Lee, H. W.;B. J. Kim;S. H. Hwang;J. Y. Kong
  15. The Second Asia-Pacific Marine Biotechnology Conference '97 and Third Asia-Pacific Conference on Algal Biotechnology, Abstract Isolation, Purification and Characterization of β-Agarase from A Marine bacterium, Bacillus cereus ASK2002 Kong, J. Y.;B. J. Kim;S. H. Hwang;S. K. Bae
  16. Kor. J. Appl. Microbiol. Biotechnol. v.19 no.4 Selection of High Tobramycin-Producing Mutants Na, K. H.;H. J. Kim;G. W. Kim;K. T. Kim;J. H. Lee;J. I. Yang
  17. Kor. J. Appl. Microbiol. Biotechnol. v.19 no.6 Effect of Some Factors on the Production of an Antifungi Compound KRF-001 from Bacillus subtilis subsp. krictiensis Son, K. H.;H. K. Kwon;H. W. Lee;S. H. Bok
  18. Kor. J. Appl. Microbiol. Bioeng. v.17 no.2 Effect of Some Parameters for Selection the High Kasugamycin Production Mutants Kim, Y. J.;S. H. Lee;K. H. Son;S. H. Bok
  19. Phaffia rhodozyma NCHU-FS501 v.16 Batch Cultivation and Astrxanthin Production by a Mutant of the red Yeast Fang, T. J.;T. Y. Chiou
  20. Kor. J. Appl. Microbiol. Biotechnol. v.23 no.1 Characterization of the Mutant of Streptomyces sp. SL-387 (KCTK 0102BP) Producing Amino peptides M Inhibitors Chung, M. C.;H. K. Chun;H. J. Lee;C. H. Lee;Y. H. Kho
  21. Enzyme Microb. Technol. v.16 Production and Selection of Mutants of Leuconostoc mesenteroides Constitutive for Glucansucrases Kim, D.;J. F. Robyt
  22. J. Biol. Chem. v.195 Notes on Sugar Determination Somogyi, M.
  23. Anal. Biochem. v.72 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding Bardford, M. M.
  24. J. Chromatog. v.49 Thin-alyer Chromatographic Analysis of Enzymic Hydrolysates of Agar Duckworth, M.;W. Yaphe
  25. J. Fement. and Bioeng. v.79 no.6 Production and Characteristics of Some New β-agarase from a Marine Bacterium, Vibrio sp. Strain JT0107 Sugano, Y.;H. Nagae;K. Inagake;T. Yamamoto;I. Terada;Y. Yamazaki
  26. Biochem. et Biophys. Acta v.537 Purification and Characterization of Two Different Agarose-Degrading Enzymes Malmqvist, M.