KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Rapid Screening of Mutant Strains of Trigonopsis variabilis (ATCC10679) for Cephalosporin C Bioconversion and Sequences of D-amino acid oxidase Genes

Cephalosporin C 생물전환을 위한 Trigonopsis variabilis (ATCC10679) 변이균주의 간편한 선별 및 D-amino acid oxidase 유전자 배열

  • 강용호 (영남대학교 응용미생물학과) ;
  • 박선영 (영남대학교 응용미생물학과)
  • Published : 1999.04.01
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Abstract

Simple and rapid screening methods were developed to screen mutant strains of Trigonopsis variabilis ATCC10679 (TW). D-amino acid oxidase (D-AAO) from a mutant strain, T26, showed about 30% higher specific activity against cephalosporin C than from its wild type, TW. D-AAO genes from both TW and T26 strains were cloned and sequenced. There was one nucleotide changed from T to C at 811 position, resulting in an amino acid codon changed from Phe-258 to Ser-258.

Trigonopsis variabilis ATCC10679 (TW)의 변이균주를 선별하기 위하여 쉽고 간편한 균주 선별방법을 개발하였다. 변이 균주(T26)의 D-Amino acid oxidase (D-AAO)는 야생균주(TW)의 D-AAO 효소보다 cephalosporin C에 대한 기질특이성이 약 30% 높았다. 이들 두 균주에서 D-AAO 유전자를 클로닝하여 각 유전자를 비교해본 결과 811번 우치의 T가 C로 변경되었으며 이에 따른 아미노산은 Phe-258이 Ser-258로 바뀌었다.

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References

  1. Biotechnol. Bioeng. v.46 Two-step immobilized enzyme conersion of cephalosporin C to 7-aminocephalosporanic acid Conlon, H. D.;J. Baqai;K. Baker;Y. Q. Shen;B. L. Wong
  2. J. Biochem. v.108 Structure and expression of cDNA for D-amino acid oxidase active against cephalosporin C from Fusarium solani Isogai, T.;H. Ono;Y. Ishitani;H. Kojo;Y. Ueda;M. Kohsaka
  3. Lett. Appl. Microbiol. v.23 D-amino acid oxidase activity from Rhodosporidium toruloides Lee, Y. H.;W. S. Chu
  4. J. Ferment. Bioeng. v.81 D-amino acid oxidase from Trigonopsis variabilis: Immobilization of whole cells in natural polymeric gels for glutaryl-7-aminocephalosporanic acid production Acunzo, A. D.;E. D. Alteriis;F. Maurano;E. Battistel;P. Parascandola
  5. Biotechnol. Lett. v.17 A process for bioconversion of cephalosporin C by Rhodotrorula gracilis D-amino acid oxidase Pilone, M. S.;S. Buto;L. Pollegioni
  6. Japan patent JP1987262994-A/1 D-amino acid oxidase gene KIenichi, K.;M. Akio;S. Kokichi
  7. Yeast v.13 Molecular cloing of TvDAO1, a gene encoding a D-amino acid oxidase from Trigonopsis variabilis and its expression in Saccharomyces cerevisiae and Kluyveromyces lactis Gonzalez, F. J.;J. Montes;F. Martin;M. C. Lopez;E. Ferminan;J. Catalan;M. A. Galan;A. Dominguez
  8. J. Biotechnol. v.58 Cloning, sequencing and expression in E. coli of a D-amino acid oxidase cDNA from Rhodotorula gracilis active on cephalosporin C Pollegioni, L.;G, Molla;S. Campaner;E. Martegani;M. S. Pilone
  9. Microbiology v.144 D-amino-acid oxidase gene from Rhodotorula gracilis (Rhodosporidium toruloides) ATCC26217 Alonso, J.;J. L. Barredo;B. Diez;E. Mellado;F. Salto;J. L. Garcia;E. Cortes
  10. Biochem. J. v.330 Chemical mechanism of D-amino acid oxidase from Rhodotorula gracilis: pH dependence of kinetic parameters Ramon, F.;M. Castillon;I. De La Mata;C. Acebal
  11. Protein Express. Purificat. v.3 Purification of Rhodotorula gracilis D-amino acid oxidase Pollegioni, L.;M. S. Pilone
  12. Enzyme Microb. Technol. v.14 Goaloriented screening method for carbohydrate oxidase produced by filamentous fungi Danneel, H-J.;M. Ullrich;F. Giffhorn
  13. Eur. J. Biochem. v.218 Evidence for the functional importance of Cys298 in D-amino acid oxidase for Trigonopsis variabilis Schrader, T.;J. R. Andreesen
  14. Arch. Biochem. Biophys. v.343 Identification of a reactive cysteine in the flavin-binding domain of Rhodotorula gracilis D-amino acid oxidase Pollegioni, L.;S. Campaner;A. A. Raibekas;M. S. Pilone
  15. Appl. Microbiol. Biotechnol. v.45 Studies on the inactivation of the flavoprotein D-amino acid oxidase from Trigonopsis variabilis Schrader, T.;J. R, Andreesen
  16. FEBS Lett. v.436 A conserved aspartate is essential for FAD binding and catalysis in the D-amino acid oxidase from Trigonopsis variabilis Ju, S. S.;L. L. Lin;W. C. Wang;W. H. Hsu
  17. Curr. Opin Struct. Biol. v.7 Structure of D-amino acid oxidase: new insights from an old enzyme Mattevi, A.;M. A. Vanoni;B. Curti