Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Studies on the Response of Photobacterium phosphoreum to the Volatile Substances

휘발성 물질에 대한 Photobacterium phosphoreum의 Bioluminescence의 변화

  • 이은수 (경희대학교 생명과학부 식품공학과) ;
  • 이용제 (경희대학교 생명과학부 식품공학과) ;
  • 김은기 (인하대학교 화공·고분자·생물공학부) ;
  • 이정건 (LG산전(주)연구소) ;
  • 전억한 (경희대학교 생명과학부 식품공학과)
  • Published : 1999.12.01
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Abstract

Various materials including sodium alginate, k-carrageenan, collagen and polyacrylamide were studied in order to maintain the stability of bioluminescence of Photobacterium for the monitoring of volatile toxic substances. Kinetic parameters of specific rate($\mu$), and gamma(${\gamma}$) value were determined for the relationship between bioluminescence of immobilized P. phosphoreum and toxic substances. The bioluminescence intensity was found to be proportional to the concentration of toxic substances and the free cells were shown to be more sensitive than immobilized cells when volatile substances were exposed to the cells. Bioluminescence increased slightly after several minutes, which was due to the volatility of toxic compounds. Furthermore, P. phosphoreum immobilized on strontium alginate was better than cells immobilized on sodium alginate for the response to substances used.

Keywords

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