Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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purification of Fungal Protease Produced by Mucor racemosus f. racemosus PDA 103 from Korean Traditional Meju

재래식 메주로부터 분리한 Mucor racemosus f. racemosus PDA 103 유래 Fungal Protease의 정제

  • 임성일 (한국식품개발연구원 생물공학연구본부) ;
  • 유진영 (한국식품개발연구원 생물공학연구본부)
  • Published : 1999.12.01
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Abstract

The protease produced by Mucor racemosus f. racemosus PDA 103 from meju was purified by precipitating with 80% saturated ammonium sulfate, CM Sephadex C-50 ion-exchange chromatography, and secondary Sephadex G-100 gel filtration chromatography. The specific activity of the purified enzyme was 60.1unit/mg protein and the purification fold of the enzyme was 83.5. The molecular weight of the enzyme was estimated 33,746Da and the enzyme was elucidated as monomer by LC-MS and SDS-PAGE. The number of amino acids was evaluated about 330 residues.

Keywords

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