Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Structure-antibiotic Acitivity Relationships of Brevinin-1 and Thanatin Containing Rana Box

Rana box를 포함한 Brevinin-1 및 Thanatin의 구조-상생활성 상관관계

  • 신송엽 (생명공학연구소, 펩타이드공학RU) ;
  • 강주현 (생명공학연구소, 펩타이드공학RU) ;
  • 이동건 (생명공학연구소, 펩타이드공학RU) ;
  • 장소윤 (생명공학연구소, 펩타이드공학RU) ;
  • 서무열 (생명공학연구소, 펩타이드공학RU) ;
  • 함경수 (생명공학연구소, 펩타이드공학RU)
  • Published : 1999.12.01
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Abstract

In order to investigate structure-antibiotic activity relationships of brevinin-1 and thanatin containing Rana box composed of basic loop formed by disulfide bridge in their arboxy terminus, thanatin, brevinin 1 and their analogues (T-B1, T-B2 and B-T) in which their Rana box sequence exchanged was designed and synthesized by the solid phase method using Fmoc-chemistry. The basic sequence of Rana box of thanatin had more significant effect on both antibacterial and antifungal activity than that of brevinin 1. The tail sequence (QRM) of thanatin was found to be important in its antibacterial and antifungal activity. Rana box sequence of brevinin-1 did not have a significant effect on its antitumor and phospholipid vesicle-aggregating activities. Brevinin-1 showed stronger $\alpha$-helical structure in the membrane-mimicking environment such as SDS micelle than thanatin. A remarkable increase in a-helicity of bervinin-1 plays more important role in antibiotic activity than that of thanatin. Furthermore, antibacterial activity of thanatin against E. coli resulted from the disruptive effect against the outer cell membrane of E. coli.

Keywords

References

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