한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제27권5호
  • /
  • Pages.419-425
  • /
  • 1999
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Bifidobacterium longum HY8001 균주의 Escherichia coli O157:H7과 Salmonella typhimurium DT104 장곤 내 감염 예방효과 및 Vero cytotoxin 중화효과

Prophylactic Effects of Bifidobacterium longum HY8001 against Escherichia coli O157:H7 and Salmonella typhimurium DT104 Enteric Infection and Evaluation of Vero Cytotxin Neutralizing Effects

  • 양수진 (서울대학교 수의과대학 미생물학교실) ;
  • 윤장원 (서울대학교 수의과대학 미생물학교실) ;
  • 서근석 (서울대학교 수의과대학 미생물학교실) ;
  • 구혜정 (서울대학교 수의과대학 미생물학교실) ;
  • 김소현 (서울대학교 수의과대학 미생물학교실) ;
  • 배형석 (한국야쿠르트 중앙연구소) ;
  • 백영진 (한국야쿠르트 중앙연구소) ;
  • 박용호 (서울대학교 수의과대학 미생물학교실)
  • 발행 : 1999.10.01
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초록

Prophylactic effects of Bifidobacterium longum HY8001, Korean isolate, against Escherichia coli O157:H7 and Salmonella typhimurium DT104 enteric infection were examined at four groups of specific pathogen free(SPF)-ICR mouse for each pathogen. B. longum HY8001+B. typhimurium DT104+B. longum HY8001(BL+ST+BL) group and B. longum HY8001+E. coli O157:H7+B. longum HY8001(BL+E+BL) group were fed with B. longum HY8001 before and after E. coli O157:H7 or s. typhimurium DT104 challenge, while B. longum HY8001+S. typhimurium DT104(BL+ST) and B. longum HY8001+e. coli O157:H7(BL+E) groups were fed with B. longum HY8001 only before E. coli O157:H7 or S. typhimurium DT104 challenge. E. coli O157:H7(E) and S. typhimurium DT104(ST) groups were challenged with each pathogen without B. longum HY8001 administration and control groups were administered with phosphate buffered solution(PBS). After the oral administration with B. longum HY8001(109cfu), th emice were challenged with E. coli O157:H7(2$\times$1010cfu) or S. typhimurium DT104(108cfu) and the mortality rate and the fecal shedding of challenged pathogen were also examined define the reactivity of the B. longum HY8001. Production of toxin neutralizing substance(s) of B. longum HY8001 was determined by cell cytotoxicity assay using Vero cells. Fecal shedding of th eS. typhimurium DT104 was significantly decreased in BL+ST+BL group fed with B. longum HY8--1 before and after challenge(p<0.05), while the fecal shedding s of S. typhimurium DT104 in BL+ST and St groups remained more than 106cfu. the protective effect of the B. longum HY8001 against E. coli O157:H7 was significantly high only in BL+E+BL group fed with b. longum Hy8001 before and after E. coli O157:H7 challenge from the result of fecal E. coli O157:H7 isolation rate, mortality rate, and intestinal contents culture to detect E. coli O157:H7. the mortality rate of the BL+e and E groups. The cytopathic effect (CPE) of the Vero cytotoxin (Shiga like toxin I & II) in Vero cell was neutralized in B. longum HY8001 culture supernatant added wells which indicate the presence of soluble Vero cytotxin neutralizing substance(s) in B. longum HY8001 culture suprnatant.

키워드

참고문헌

  1. Trends in Microbiol. v.6 no.3 Probiotics, prebiotics or conbiotics Berg, R. D.
  2. Appl. Environ. Microbiol. v.64 no.5 Effect of dietary stress on fecal shedding of Escherichia coli O157:H7 in calves Cray, W. C.;Jr.,T. A. Casey;B. T. Bosworth;M. A. Rasmussen
  3. Appl. Environ. Microbiol. v.63 no.2 Proteinaceous factors in culture supernatant fluids of bifidobacteria which prevents the binding of enterotoxigenic Escherichia coli to gangliotetraosylceramide Fujiwara, S.;H. Hashiba;T. Hirota;J. F. Forstner
  4. J. Darity Sci. v.74 Comparison of probiotic and antibiotic intramammary therapy of cattle with evaluated somatic cell counts Greene, W. A.;A. M. Gano;K. L. Smith;J. S. Hogan;D. A. Todhunter
  5. Am. J. clin. Nutr. Review v.125 Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics Gibson, G. R.;M. B. Roberfroid
  6. MMWR v.46 Multidrug resistant Salmonella serotype Gosek, G.;D. Leschinsky;S. Irons (et als.)
  7. J. Appl. Bacteriol. v.32 Effects of food intake on numbers of Salmonella and Escherichia coli in rumen and feces of sheep Grau, F. H.;L. E. Brownlie;M. G. Smith
  8. Salmonella typhimurium DT104. Situation Assessment Hogue, A.;J. Akkina;F. Angulo (et als.)
  9. Infect Immun v.66 no.4 Enhancement of susceptibility to Shiga toxin-producing Escherichia coli O157:H7 by protein calorie malnutrition in mice Kurioka, T.;Y. Yunou;E. Kita
  10. J. Clin. Microbiol. v.34 Escherichia coli O157:H7 in microbial flora of sheep Kudva, I. T.;P. G. Hatfield;C. J. Hovda
  11. J. Appl. Bacteriol. v.79 In vitro inhibition of Helicobacter pylori NCTC 11637 by organic acid and lactic acid bacteria Midolo, P.D.;J. R. Lambert;R. Hull;F. Luo;M. L. Grayson
  12. J. Dairy Sci. v.78 Symposium: Probiotic bacteria for humans: Clinical systems for evaluation of effectiveness Perdigon, G.;S. Alvarez;M. Rachid;G. Aguero;N. Gobbato
  13. Clinical Microbiology Reviews v.11 no.3 Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections Patron, J. C.;A. W. paton
  14. Appl. Environ. Microb. v.59 no.9 Trypsin-dependent production of an antibacterial substance by human Peptostreptococcus strain in gnotobiotic rats and in vitro Ramare, F.;J. Nicoli;J. Dabard;T. Corring;M. Ladire;A. M. Gueugneau;P. Raibaud
  15. Bergey's Manual, section 15 Bifidobacterium Scardovi, V. Genus
  16. Molecular Genetics of Bacteria Snyder, L.;W. Champness
  17. Poultry Science v.62 Competitive gut exclusion of Avian pathogens by Lactobacillus acidophilus in gnotobiotic chicks Watkins, B. A.;B. F. Miller
  18. J. Clinical Microbiol. v.36 no.3 Reduction of carriage of enterohemorrhagic Escherichia coli O157:H7 in cattle by inoculation with probiotic bacteria Zhao, T.;M. P. Doyle;B. G. Harmon;C. A. Brown;P. O. Eric Mueller;A. H. Parks