Methanol 자화방선균 MO-16으로부터 항균성 물질의 정제 및 생산조건

Purification and Production Conditions of Antimicrobial Compound from Methylotrophic Actinomycetes MO-16

  • 김현수 (계명대학교 자연과학대학 미생물학과) ;
  • 이정수 (계명대학교 자연과학대학 미생물학과)
  • 발행 : 1999.10.01

초록

A methylotrophic actinomycetes strain MO-16, which produce the antimicrobial compound, was isolated from soil and supposed as Amycolatopsis sp. based on taxonomic studies. The cell-free extract of methanol-grown strain MO-16 showed dehydrogenase activity for methanol and formaldehyde when various electron acceptors were added for oxidation. On the other hand, methanol did not affect the production of antimicrobial compounds, and organic nitrogen sources such as corn steep liquor and peptone were better than inorganic nitrogen sources. These compounds showed broad antimicrobial spectrum to the tested strains such as bacteria and yeast. The antimicrobial comounds were very stable under heat(121$^{\circ}C$), acid(pH2.0), alkali(pH11.0) treatments. These compounds were isolated by ethylacetate extract, silica gel column chromatography and reverse phase HPLC. Two compounds(peak 1 and 2) were detected as antimicrobial compounds through the HPLC analysis. The peak 2 was purified as a single compound and revealed a 98% purity.

키워드

참고문헌

  1. Eur. J. Biochem. v.247 Tetrazolium-dye-linked alcohol dehydrogenase of the methylotrophic actionmycete Amycolatopsis methanolica is a three-component complex Bystrykh, L. V.;N. I. Govoruchina;L. Dijkhuizen;J. A. Duine
  2. Arch. Microbiol. v.153 Regulation of methanol metabolism in the facultative methylotroph sp. 239 during growth on mixed substrates in batch and continuous cultures De boer, L.;G. J. Euverink;J. van der Vlag;L. Dijkhuizen
  3. Int. J. Syst. Bacteriol. v.40 Amycolatopsis methanolica sp. nov., a facultatively methylotrophic actinomycete De Boer, L.;L. Dijkhuizen;G. Grobben;M. Goodfellow;E. Stackebrandt;J. H. Parlett;D. Whitechead;D. Witt
  4. FEBS Lett. v.168 NAD-dependen PQQ-containing methanol dehydrogenase: A bacterial dehydrogenase in multi-enzyme complex Duine, J. A.;J. Frank;M. P. J. Berkhout
  5. Experimental Methods of Indentification of Actinomycetes(in Japanese) Hamada, M.;M. Manabe
  6. Arch. Microbiol. v.135 Nocardia sp. No. 239, a facultative methanol utilizer with the ribulose monophosphate pathway of formaldehyde fixation Hazeu, W.;J. C. de Bruyn;J. P. van Dijken
  7. J. Ferment. Technol. v.52 A methanol-utilizing actinomycetes Kato, N.;K. Tsuji;Y. Tani;K. Ogata
  8. Microbial C1-compounds Kato, N.;K. Tsuji;Y. Tani;K. Ogata
  9. Arch. Biochem. Biophys. v.325 A second molybdoprotein aldehyde dehydrogenase from Amycolatopsis methanolica NCIB 11946 Kim, S. W.;D. M. A. M. Luykx;S. de Vries;J. A. Duine
  10. J. Inst. Nat. Sci. v.15 Isolation and production of antifungal compound from methylotrophic actinomycetes Kim, H. S.
  11. Korean J. Biotechnol. Bioeng. v.12 Production of aminoglycoside-resistant inhibitor from methylotrophic actinomycetes Kim, H. S.;J. O. Shin;T. S. Yu
  12. Actinomycetol v.3 A new method for intensive isolation of actinomycetes from soil Masayuki H.;H. Nomomura
  13. Atlas of Actinomycetes Miyadoh, S.;M. Hamada;K. Hotta;T. Kudo;A. Seino;G. Vobis;A. Yokota
  14. J. Ferment. Technol. v.65 Purification and characterization of methanol dehydrogenase of a serine producing methylotroph, Hyphomicrobium methlovorum Miyazak, S. S.;J. Toki;Y. Izumi;H. Yamada
  15. Int. Microbiol. and Food Ind. Congr. Paris Production of scp by methanol utilizing microorganisms Sahm, H.
  16. Hakkokoguku Kaishi v.56 Microbial utilization of methanol Tani, Y.;N. Kato;Y. Izumi;S. Shimizu;K. Ogata;H. Yamada
  17. FEMS Microbiol. Lett. v.80 A novel dye-linked alcohol dehydrogenase activity present in some Gram-positive bacteria Van Ophem, P. W.;G. J. Euverink;L. Dijkhuizen;J. A. Duine
  18. Eur. J. Biochem. v.212 Nicotinoprotein [NAD(P)-containing] alcohol/ aldehyde oxidoreductases purification and characterization of a novel type from Amycolatopsis methanolica Van Ophem, P. W.;J. Van Beeumen;J. A. Duine
  19. Eur. J. Biochem. v.206 Dye-linked dehydrogenase activities for formate and formate esters in Amycolatopsis methanolica. Characterization of molybdoprotein enzyme active with formate esters and aldehyde Van Ophem, P. W.;L. V. Bystrych;J. A. Duine
  20. Bergey's Manual of Systematic Bacteriology v.4 Williams, S. T.;M. E. Sharpe;J. G. Holt
  21. Agric. Biol. Chem. v.41 Purification, crystallization and properties of primary alcohol dehydrogenase from a methanol-oxidizing Pseudomonas sp. No. 2941. Yamaka, K.;K. Matsumoto
  22. J. Ferment. Technol. v.65 Improvement of cytochrome c production by glycin and glycin analog resistant mutants of Methylomonas sp. Yoon, B. D.;M. Ueno;Y. Tani