한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제27권3호
  • /
  • Pages.203-207
  • /
  • 1999
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

해양 호염성 세균 Vibrio alginolyticus가 생산하는 Extracellular Amylase의 특성

Properties of an Extracellular Amylase Produced by the Marine Halophilic Bacterium Vibrio alginolyticus

  • 김영재 (창원대학교 자연과학대학 미생물학과)
  • 발행 : 1999.06.01
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초록

V. alginolyticus 138-2, a marine halophilic bacterium, produced an extracellular amylase with a molecular weight of ca. 56,000. The analysis of the digestion products of soluble starch by thin layer chromatography(TLC) revealed that the extracellular amylase of V. alginolyticus 138-2 is a saccharifying-type alpha-amylase. The alpha-amylase activity of the culture supernatant of soluble starch was optimal at pH 6.0 and 45$^{\circ}C$. Ca2+ slightly increased the alpha-amylase activity, whereas Hg2+, An2+, Cu2+, Ni2+, Fe2+, and Mn2+inhibited the enzymatic activity. Alkylating thiol group agent, iodoacetic acid did not affect the alpha-amylase activity, but reduced thiol reagents such as dithiothreitol, cysteine, and beta-mercaptoethanol stimulated theenzymatic activity. On the other hand, even if V. alginolyticus 138-2 is a marine halophilic bacterium, its alpha-amylase activity was significantly inhibited by NaCl.

키워드

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