Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Mass Cultivation of Botryococcus braunii for the Advanced Treatment of Swine Wastewater and Lipid Production in a Photobioreactor

축산폐수의 고도처리 및 지질생산을 위한 Botryococcus branuii의 대량배양

  • Published : 1999.04.01
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Abstract

This study was conducted to investigate the removal rate of nitrogen and phosphorus, and lipid production from a swine wastewater by Botryococcus braunii UTEX 572 in an outdoor photobioreactor. B. braunii successfully predominated in competition with bacteria and other algae, especially Oscillatoria, which were grown spontaneously in a secondary-treated swine wastewater, under the conditions of incubation temperature at $25^{\circ}C$ and increased inoculum amount at 287mg/l. There was a significant relationship between dry weight of B. braunii and absorbance of culture solution at 680mn(r2=0.967), suggesting that the latter is as good as the former commonly used for the measurement of algal biomass which is considerably time-consuming. The removal rates of COD, TOC, total nitrogen, and total phosphorus from the swine wastewater were 33.2$\pm$2.6% and 32.8$\pm$3.2, respectively, which showing no different between them. These results suggested that the mass cultivation of B. braunii in an outdoor photobioreactor could be used for the advanced treatment of swine wastewater and lipid production.

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References

  1. Water Sci. Technol. v.28 Industrial Wastewater treatment using an activated algae-reactor Aziz, M. A.;W. J. Ng
  2. Algal Biotechnology Development of microalgal systems for the production of liquid fuels Barclay, W.;C. Wyman;R. A. Lewin;L. Cheng;Stadler(ed.)
  3. Biotech. Bioeng. v.27 Studies on batch and continuous cultures of Botryococcus braunii: Hydrocarbon production in relation to physiological state, cell ultrastructure, and phosphatc nutrition Casadevall, E.;D. Dif;C. Largeau;C. Gudin;D. Chaumont;O. Desanti
  4. Appl. Microbiol. Biotechnol. v.29 Biological tertiary treatment of urban wastewaters with chitosan-immobilized Phormidium De la Noue, J.;D. Proulx
  5. Biological Wastes v.29 Biotreatment of anaerobically digested swine manure with microalgae De la Noue, J.;A. Basseres
  6. Limnol. Oceanogr. v.22 Determination of total nitrogen in aqueous samples using persulfate digestion D'Elia, C. F.;P. A. Steudler;N. Corwin
  7. Ambio. v.19 Ecological engineering for wastewater treatment and its application in new England and Sweden Guterstam, B.;J. Todd
  8. Standard Methods for Environmental Pollution Kim, J. T.
  9. Kor. J. Appl. Microbiol. Biotechnol. v.26 Selection of microalgae for advanced treatmetn of swine wastewater and optimization of treatment condition Kim, S.-B.;S. J. Lee;C.-K. Kim;G.-S. Kwon;B.-D. Yoon;H.-M. Oh
  10. FEMS Microbiol. Rev. v.16 Liquid and gaseous fuels from biotechnology: Challenge and opportunities Kosaric, N.;J. Velikonja
  11. Biotechnol. Tech. v.12 Rapid method for the determination of lipids from the green alga Botryococcus braunii Lee, S. J.;B.-D. Yoon;H.-M. Oh
  12. Lett. Appl. Microbiol. v.27 Effects of harvesting time and growth stage on the flocculation of the green alga Botryococcus braunii Lee, S. J.;S._B. Kim;J.-E. Kim;G.-S. Kwon;B.-D. Yoon;H.-M. Oh
  13. Analyt. Chem. Acta v.27 A modified single solution method for the determination of phosphate in natural waters Murphy, J.;J. P. Riley
  14. Kor. J. Appl. Microbiol. Biotechnol. v.25 Effects of light intensity and nutrients on the growth of Botryococcus sp. Oh, H.-M.;S.-B. Kim;J.-H. Park;E.-R. Park;S.-T. Lee;G.-S. Kwon;B.-D. Yoon
  15. Appl. Biochem. Biotech. v.67 Hydrocarbon production by the Yayoi, a new strain of the green microalga Botryococcus braunii Okada, S.;M. Murakami;K. Yamaguchi
  16. Algae v.13 Advanced treatment of swine wastewater by a green alga, Scenedesmus quadricauda Park, M.-K.;S. J. Lee;H.-H. Suh;H.-S. Kim;Y. H. Kim;B.-D. Yoon;H.-M. Oh
  17. Kor. J. Biotechnol. Bioeng. v.10 Kinetics of cultivating photosynthetic microalgae, Spirulina platensis in an outdoor photobioreactor Sung, K.-D.;J.-H. Ann;J.-Y. Lee;S.-J. Ohh;H.-Y. Lee
  18. Phytochemistry v.23 Mechanism of non-isoprenoid hydrocarbon biosynthesis in Botryococcus braunii Templier, J.;C. Largeau;E. Casadevall
  19. Appl. Biochem. Biotech. v.8 Botryococcus braunii: An unusual hydrocarbon-producing alga Wolf, F. R.
  20. J. Phycol. v.21 Growth and branched hydrocarbon production in a strain of Botryococcus braunii(Chlorophyta) Wolf, F. R.;A. M. Nonomura;J. A. Bassham
  21. Can. J. Fish Aquat. Sci. v.42 Chloroform-methanol extraction of chlorophyll a Wood, L. W.
  22. Appl. Biochem. Biotech. v.39/40 Biotechnology for production of fuels, chemicals, and materials from biomass Wyman, C. E.;B. J. Goodman
  23. J. Plankton Res. v.8 Effects of light intensity and quality on the relative N and P requirement (the optimum N:P ratio) of marine planktonic algae Wynne, D.;G-Y. Rhee
  24. Agric. Biol. Chem. v.51 Lipid composition of a green alga, Botryococcus braunii Yamaguchi, K.;H. Nakano;M. Murakami;S. Konosu;O. Nakayama;M. Kanda;A. Nakamura;H. Iwamoto