Pseudomonas aeruginosa JRT-4에 의해 생산된 Biosurfactant의 계면활성 및 환경적 특성

Surface-activity and Environmental Characteristics of Biosurfactant Produced by Pseudomonas aeruginosa JRT-4

  • 황경아 (LG화학 생활과학연구소) ;
  • 이정래 (LG화학 생활과학연구소) ;
  • 김상종 (서울대학교 미생물학과) ;
  • 김윤석 (LG화학 생활과학연구소) ;
  • 안호정 (LG화학 생활과학연구소)
  • 발행 : 1999.04.01

초록

Pseudomonas aeruginosa JRT-4 strain was used as a biosurfactant-producing microorganism in this study. It was one of the microorganisms isolated from the sewage sludge, the main and branch streams of Han river. The surface tension of the culture broth of P. aeruginosa JRT-4 decreased to 30mN/m. The crude biosurfactant was obtained from the culture broth by acid precipitation, solvent extraction, evaporation, and freeze drying. The CMC value of the crude biosurfactant was 0.006%(w/v). From analysis of the chemical structure of biosurfactant, it was determined as rhamnolipid 1 and 3 structures by FAB mass spectrometer. In the washing test for artificially contaminated textiles, the biosurfactant showed better bleachness than the two chemically synthesized surfactant, LAS and SLES. Finally, the biodegradation and ecotoxidolorical tests showed that the biosurfactant was readily biodegradable in the environment and a mild material for microorganisms and green algae.

키워드

참고문헌

  1. Microbiol. Sci. v.3 Biosurfactants Cooper, D. G.
  2. J. Sci. Ind. Res. v.46 Microbial surfactants: Evaluation, types and future applications Desai, J. D.
  3. Biosurfactants: Production, Properties, Applications Production of biosurfactants Desai, J. D.;A. J. Desai;N. Kosaric(ed.)
  4. Biotechnol. Bioeng. v.24 Production of surfactant by Arthrobacter paraffineus Aicc 19558 Duvnjak, Z.;D. G. Cooper;N. Kosaric
  5. Chem. Eng. News v.69 Sales of detergents growing despite recession Greek, B. F.
  6. Agric. Biol. Chem. v.35 Formation of rhamnolipid by Pseudomonas aeruginosa: Its function in hydrocarbon fermentations Hisatsuka, K.;T. Nakahara;N. Sano;K. Yamada
  7. Testing method for biodegradability of synthetic detergent KS
  8. Korean J. Limnol. v.26 Biodegradation of anionic surfactants in continuous activated sludge, semicontinuous sludge and shake culture Lee, M. H.;D. W. Baek;J. R. Lee;Y. K. Kim
  9. J. Ind. Microbiol. Biotechnol. v.18 Biosurfactant production by a thermophilic Bacillus subtilis strain Makkar, R. S.;S. S. Cameotra
  10. Senzai Senjounojiten Minakawa, M.
  11. Guideline for testing of chemicals OECD
  12. J. Am. Oil. Chem. Soc. v.66 Chemical characterization and physicochemical behaivor of biosurfactants Parra, J. L.;J. Guinea;M. A. Manresa;M. Robert;M. E. Mercade;F. Comelles;M. P. Bosch
  13. Appl. Microbiol. Biotechnol. v.29 Biosurfactant production by Pseudomonas fluorescens 378: Growth and product characteristics Persson, A.;E. Oesterberg;M. Dostalek
  14. Biotechnol. Lett. v.11 Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T Robert, M.;M. E. Mercade;M. P. Bosch;J. L. Parra;M. J. Espuny;M. A. Manresa;J. Guinea
  15. Soap Cosmet. Chem. Specialities v.70 Surfactants-94 Shaw, A.
  16. Chemosphere v.28 Development and evaluation of a growth inhibition test with sewage bacteria for assessing bacterial toxicity of chemical compounds Strotmann, U. J.;H. Eglsaer;U. Pagga
  17. Biosurfactants and Biotechnology Production of biosurfactants Syldatk, C.;F. Wagner;N. Kosaric(ed.);W. L. Cairns(ed.);N. C. C. Gray(ed.)