Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimization of the Condition of Immobilized Photobacterium phosphoreum with Strontium Alginate

Strontium Alginate를 담체로 한 Photobacterium phosphoreum 고정화 조건의 최적화

  • 이홍주 (경희대학교 생명자원과학부 식품가공학과) ;
  • 김현숙 (경희대학교 생명자원과학부 식품가공학과) ;
  • 정계훈 (경희대학교 생명자원과학부 식품가공학과) ;
  • 이은수 (경희대학교 생명자원과학부 식품가공학과) ;
  • 전억한 (경희대학교 생명자원과학부 식품가공학과)
  • Published : 1999.04.01
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Abstract

Since the condition of immobilization must be optimized, it is very important to know whether and on how conditions bacterial cells retain their metabolic activity during immobilization process. A bioluminescence intensity had the maximum value when cell concentrations were between 1.0 and 1.2 measured at O.D660. The strontium alginate was used as an immobilization matrix and two independent factors for immobilization of Photobacterium phosphoreum with strontium alginate were optimized with the response surface methodology(RSM) considering degree of bioluminescence. As a result, the optimum concentration for immobilization was found to be 2.4%(w/w) for sodium alginate and 0.31M for strontium chloride, respectively. A dilution was carried out with 2.5%(w/v) NaCl solution that is an optimum environmental condition for growth of P. phosphoreum. Under the such condition of immobilization, hardness could be predicted as 4.66$\times$104N/$m^2$ and it took different time according to the volume of matrix to be immobilized completely.

Keywords

References

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