Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Chemopreventive Effect of Gamdutang Aqua-Acupuncture Solution

감두약침액의 암예방 효과

  • 한상훈 (동국대학교 한의과대학 경혈학교실) ;
  • 조경희 (동국대학교 한의과대학 경혈학교실) ;
  • 최혜경 (동국대학교 한의과대학 경혈학교실) ;
  • 임종국 (동국대학교 한의과대학 경혈학교실) ;
  • 손윤희 (의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 이임태 (의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 남경수 (의과대학 약리학교실 및 난치병한양방치료연구센터)
  • Published : 1999.12.01
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Abstract

Gamdutang aqua-acupuncture solution (GAS), Gamdutang water-extracted solution (GWS) and Dae-Gamdutang aqua-acupuncture solution (DGAS) were prepared and tested for chemopreventive potentials. GAS was potent inducer of quinone reductase (QR) activity in Heapa1c1c7 murine hepatoma cells in culture, whereas GWS is less potent. GAS, GWS and DGAS were significantly induced QR activity in cultured rat normal liver cell, Ac2F. Glutathione (GSH) levels were increased about 1.8, 1.0 and 1.1 fold with GAS, GWS and DGAS in Hepa1c1c7 cells, respectively. In addition glutathione S-transferase (GST) activity was increased with GAS, GWS and DGAS. The effects of GAS, GWS and DGAS on the growth of Acanthamoeba castellanii were tested. Proliferation of A. castellanii was inhibited by GAS, GWS and DGAS at concentrations of 1 $\times$ and 5$\times$. These results suggest that GAS has chemopreventive potential by inducing QR and GST activities, increasing GSH levels and inhibition of polyamine metabolism.

한방에서 염증, 종양 억제 등에 사용되는 감초와 흑소두, 감초와 흑대두를 정량으로 배합하여 조제한 약침액을 이용하여 암 억제효과를 살펴 보았다. 발암물질을 무독화 시키는 QR 생성 유도를 생쥐의 간암세포인 Hepa1c1c7과 rat의 간세포 Ac2F에서 측정하였으며, 그 결과 감두약침액, 열수추출액을 처리한 Hepa1c1c7 세포에서 감두약침액과 열수 추출액에 의한 GSH 생성이 증가하였고, GST활성측정에 의하면 Hepac1c7 세포에서 감두약침액에 의한 GST 생성이 증가하였다, 농도별 감두약침액과 역수추출액에 의한 A. castellanii 증식을 살펴 본 결과, 약침수액과 열수추출액이 A. castellanii 증식을 농도 의존적으로 저해하는 효과가 있었다.

Keywords

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