채소작물의 형질전환을 위한 재분화체계 확립 및 항생제 검정

Establishment of Regeneration System and Antibiotic Sensitivity Test for Transformation of Various Vegetable Crops

  • 발행 : 1999.10.01

초록

This study was conducted to determine the concentrations of plant growth regulators required for regeneration and the concentrations of antibiotics for the selection of transformed regenerants from lettuce, musk melon and tomato. The optimal concentrations of plant growth regulators for shoot formation were NAA 0.1 mg/$\ell$ +BA 0.1 mg/$\ell$ for lettuce, NAA 0.01 mg/$\ell$ +BA 2.0 mg/$\ell$ for melon and NAA 0.1 mg/$\ell$ +BA 0.5 mg/$\ell$for musk melon. Shoot induction from tomato, lettuce and melon was completely inhibited by 30 mg/$\ell$ or higher concentrations of kanamycin. Shoot formation from mu나 melon was not affected by kanamycin up to 40 mg/$\ell$, but was reduced in the presence of 50 mg/$\ell$ and completely inhibited by 100 mg/$\ell$. Shoot formation of all four crops was completely inhibited by higromycin at 10 mg/$\ell$. Both carbenicillin and cefatoxinme did not show any negative effects on shoot formation.

키워드

참고문헌

  1. Gene. v.19 Nucleic acid sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn. Beck,E.;Ludwig, G.;Auerswald, E.A.;Reiss, B.;Schaller,H.
  2. Plant Cell. Rep. v.12 Agrobacterium-mediated transformation of Asparagus officinals L. Iong-term. embryogenic calls and regeneration or transgenic plants Bruno, D. ;Philippe, G.;Jullien, M.
  3. EMBO. J. v.13 An Agobacterium-transformed cell culture from the monocot Asparagus officnals Hernalsteens, J.P.;Thia-Tong,L.;Schell, J.;van Montagu. M.
  4. EMBO. J. v.4 Chimeric genes as dominant seletable makers in plant cells Herrera-Estrella,L.;De Block, M.;Messenes, E.;Hernalsreens, J.P.;van Montague; M.;Shell, J.
  5. Plant. Mol. Biol. v.7 Bleomycin resistance;a new dominant selectable marker for plant cell transformation Hille, J.;Verheggen, F.;Roelvink, P.;Frassen, H.;van Kammen, A.;Zabel, P.
  6. Mol. Gen. Genet. v.210 A dominant nuclear streptomycin resistance maker for plant cell transformation Jones. J.D.G.;Svab, Z.;Harper,E.C.;Hurwitz, C.D.;Maliga. P.
  7. Nucleic Acids Res. v.11 Analysis of a bacterial hygromycin B resistance gene by transcription and translation fusions and by DNA sequencing Kaster, K.R.;Burgett,S.G.;Rao, R.N.;Ingolia, T.D.
  8. Horticulture Abst v.14 no.1 Efficient Agobacterium-mediated transformation of hot pepper(Capsicum annuumL.);The Korean Society for Horticultural Science Kim, D.S.;Kang,B.C.;Kim, K.T.;Oh, D.G.;Yoon, J.Y.
  9. MS thesis, Seoul National University Studies on in vitro regeneration and transformation of hot pepper(Capsicum annuum L.) Maria,B.H.;Medford, J.I.;Hoffman, N.L.;Rogers,S.T.;Klee, H.J.
  10. Plant. Physiol. v.86 Development of a plant transformation selection system based on expression of genes encoding gentamycin acetyltransferase Maria, B.H.;Medford,J.I.;Hoffman, N.L.;Rogers, S.T.;Klee,H.J.
  11. L. Em. Thell. Plant. Sci. v.46 Cefatoxime stimulates callus growth embryogenesis and regeneration in hexaploid bread wheat Triticum asetivum Mathias, R.J.;Boyd,L.A.
  12. Plant. Cell Rep. v.6 The effect of cefataxime on the growth and regeneration of callus from varieties of barley (Hordeum vulgareL.) Mathias,R.J.;Musaka, C.
  13. Amer. Potato J. v.72 Plant regeneration from leaf tissue of four North Dakota Genotypes of potato(Slanum tuberosum L.) Park, Y.D.;Ronis,D.H.;Boe, A.A. ;Cheng, Z.M.
  14. Plant Cell Rep. v.2 The toxicity of antibiotics to plant cell cultures Pollock,K.;Barfield, D.G.;Shields, R.
  15. Plant Cell.Rep. v.11 Transformation and regeneration of Brassicarapa using Agrobacterium tumefaciens Sharon,E.;Joann, C.R. ;Turner,C.;Daniel,F.
  16. Plant Cell Tissue Organ Cult. v.21 In vitro shoot regeneration from leaf discs of pendula "Dalecarlica" EM85. Valobra, C.P.;James,D.J.
  17. Plant Mol. Biol. v.5 Resistance to hygromycin B. A new marker for palnt transformation Waldron, C.;Murphy,E.B.;Roberts, J.L.;Gustafson, G.D.;Amour, S.L.;Malcolm, S.K.