생명과학회지 (Journal of Life Science)

  • 제9권4호
  • /
  • Pages.466-472
  • /
  • 1999
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

동물실험을 통한 솔잎(松葉) 유효성분의 항노화효과 구명 및 구조 해명 I. 간장의 활성산소 및 제거효소에 미치는 솔잎 추출물의 영향

Investigation of Anti-aging Effect and Determination of Chemical Structures of Pine Needle Extract (PNE) through the Animal Experiments I. Effects of PNE on Oxygen Radicals and Their Scavenger Enzymes in Liver of SD Rats

  • 최진호 (부경대학교 식품생명공학부 생화학교실) ;
  • 김대익 (부경대학교 식품생명공학부 생화학교실) ;
  • 박수현 (부경대학교 식품생명공학부 생화학교실) ;
  • 김동우 (부경대학교 식품생명공학부 생화학교실) ;
  • 이종수 (부경대학교 식품생명공학부 생화학교실) ;
  • 김현숙 (숙명여자대학교 식품영양학과)
  • 발행 : 1999.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

These studies were designed to investigate the effects of pine (Pinus densiflora Sieb et Zucc.) needle extract (PNE) on oxygen radicals and their scavenger enzymes in liver membranes of Sprague-Dawley (SD) rats as a study on investigation of anti-aging effect and determination of chemical structures of PNE through the animal experiments. Male SD rats were fed basic diets (control group) and experimental diets (0.5% and 1.0%-PNE group) for 6 weeks. There were no significant differences in hydroxyl radical (·OH) formations of liver mitochondria and microsomes in 0.5%-PNE group, while ·OH formations were significantly decreased (10% and 18%, respectively) in liver mitochondria and microsomes of 1.0%-PNE group compared with control group. Microsomal hydrogen peroxides and cytosolic superoxide radicals were remarkably decreased (20% and 20∼25%, respectively) in 0.5% and 1.0%-PNE groups compared with control group. Mn-SOD activities in mitochondria were significantly increased about 10% in 1.0%-PNE group, while Mn-SOD activities in mocrosomes were remarkably increased (16∼20%) in 0.5% and 1.0%-PNE groups compared with control group. There were no significant differences in Cu, Zn-SOD activities of liver cytosol in 0.5% and 1.0%-PNE groups, while glutathione peroxidase (GSHPx) and catalase (CAT) activities were significantly decreased (28∼30% and 15∼30%, respectively) in liver cytosols of 0.5% and 1.0%-PNE groups compared with control group. These results suggest that these PNE may play a effective role in a attenuating a oxygen radical formations and increasing a scavenger enzyme activities.

키워드

참고문헌

  1. Chem. J. Biol v.249 Enzyme catalyzed free radical reactions with nicotinamide adenine nucleotide Chan, P. C.;Bielski, B. H. T.
  2. Age v.12 The effect of food restriction on kidney membrane structures of aging rats Choi, J. H.;Yu, B. P.
  3. Age v.13 Unsuitability of TBA test as a lipid peroxidation marker due to prostaglandin synthesis in the aging kidney Choi, J. H.;Yu, B. P.
  4. Kor, J. Biochem v.23 no.1 Lipid peroxidation, aging and food restriction Choi, J. H.
  5. J. Kor. Soc. Food Nutr. v.20 no.2 Modulation of the aging process by food restriction Choi, J. H.
  6. Free Rad. Biol. & Med. v.18 no.2 Brain synaptosomal aging : Free radicals and membrane fluidity Choi, J. H.;Byung P. Yu.
  7. Kor. J. Gerontol v.4 no.2 Studies on agerelated physiological changes in brain of senescence accelerated mouse(SAM) Choi, J. H.;Yu, B. P.
  8. Age v.19 Analysis of lipid composition and hydroxyl radicals in brain membranes of senescence-accelerated mice Choi, J. H.;Kim, J. I.;Kim, D. W.;Moon, Y. S.;Chung, H. Y.;Yu, B. P.
  9. J. Nutr. Health & Aging v.2 no.3 The effects of dietary restriction on age-related changes in rat serum prostaglandins Choi, J. H.;Yu, B. P.
  10. J. Nutr. Health & Aging v.2 no.3 Dietary restriction as a modulator of age-related changes in rat kidney prostagladin production Choi, J. H.;yu, B. P.
  11. J. Nutr. Health & Aging v.2 no.3 Modulation of age-related alterations of iron, ferritin, and lipid peroxidation in rat synaptosomes Choi, J. H.;Kim, D. W.;Yu, B. P.
  12. Age & Nutrition v.10 no.1 The effects of dietary restriction on age-related changes in rat serum prostaglandins Choi, J. H.;Yu, B. P.
  13. Korean J. Life Sci. v.7 no.4 Effect of pine needle extract (PNE) on physiological activity of SD rats I. Feeding effect of PNE on lipid and oxygen radical metabolisms in serum of SD rats. Choi, J. H.;D. W. Kim;J. H. Kim;K. S. Kim;J. S. Lee
  14. Korean J. Life Sci. v.8 no.1 Effect of pine needle extract (PNE) on physiological activity of SD rats. II. Feeding effect of PNE on oxygen radicals and their scavenger enzymes in brain membranes of SD rats. Choi, J. H.;J. H. Kim;D. W. Kim;K. S. Kim;J. S. Lee
  15. Korean J. Life Sci. v.8 no.2 Effect of pine needle extract (PNE) on physiological activity of SD rats. III. Feeding effect of PNE on fluidity and neurotransmitter-related enzymes in brain membranes of SD rats. Choi, J. H.;J. H. Kim;D. W. Kim;C. H. Hwang;J. S. Lee
  16. FEBS Lett. v.128 Formation of a thiobarbituric acid-reactive substance from deoxyribose in the persence of iron salts. Halliwell, B.;Gutteridge, J. M. C.
  17. J. Gerontol v.11 Aging : a theory based on free radical and radiation chemistry Harman, D.
  18. J. Korean Soc. Food Nutr. v.25 no.3 Effects of pine needle extracts on serum and liver lipid contetns in rata fed high fat diet Kang, Y. H.;Y. K. Park;T. Y. Ha;K. D. Moon
  19. J. Korean Soc. Food Nutr. v.25 no.3 Effects of pine needle extracts on enzyme activities of serum and liver, and liver morphology in rats fed high fat diet Kang, Y. H.;Y. K. Park;T. Y. Ha;K. D. Moon
  20. Korean J. Gerontol v.1 no.1 Effect of dietary supplementation with pine leaf on lipid parameters in rats Kim, J. D.;T. H. Yoon;N. Choi;K. J. Im;J. S. Ju;S. Y. Lee
  21. Mutat. Res. Aug. v.347 no.3-4 Study on the antimutagenic effect of pine needle extract Kong, Z.;Liu, Z.;ding, B.
  22. Lipid v.19 Species, tissue and subcellular distribution of non-Se-dependent glutathione peroxidase activity Lawrence, R. A.;Burk, R. F.
  23. J. Biol. Chem. v.193 Protein measurement with the Folin-Phenol reagent Lowry, O. H.;Roseborough, N. J.;Farr, L. A.;Randall, R. J.
  24. Chem. J. Biol. v.244 Superoxide dismutase. An enzymic function for erythro cuprein (hemo cuprein) McCord, J. M.;Fridovch, I.
  25. Anal. Biochem v.42 Reevaluation of assay methods and establishment of kit for superoxide dismutase activity Oyanagui,Y.
  26. Anal. Biochem. v.81 Simultaneous determination of superoxide dismutase and catalase in biological materials by polarogtaphy Rigo, A.;Rotilio, G.
  27. Principles and procedures of stastistics Steel, R. G. D.;Torrie, j. H.
  28. Eur. J. Biochem v.25 Hepatic microsomal ethanol oxidation Thurman, R. G.;Ley, h. G.;Scholz, R.
  29. Soc. Exp. Biol. Med. v.193 Mechanism of food restriction: Protection of cellular homeostasis Yu, B. P.;Lee, D. W.;marler, C. G.;Choi, J. H.
  30. Free Rad. Biol. Med. v.21 Aging and oxidative stress: Modulation by dietary restriction Yu, B. P.
  31. Ann. N. Y. Acad. Sci. v.786 Critical evaluation of free radical theory of aging: A proposal of oxidative stress hypothesis Yu, B. P.;Yang, R.
  32. 韓國食經大典 李盛雨
  33. 原色和漢藥圖鑑(下) 難波恒雄