생명과학회지 (Journal of Life Science)

  • 제9권4호
  • /
  • Pages.453-459
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  • 1999
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

보리새우류의 바이러스 감염증의 진단

Diagnosis of Viral Infection of Pensaeide

  • 허문수 (제주대학교 해양과학대학 해양생산과학부) ;
  • 정초록 (부산대학교 자연과학대학 생명과학부) ;
  • 장경립 (부산대학교 자연과학대학 생명과학부)
  • 발행 : 1999.08.01
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초록

Baculovirus(WSBV) was isolated from infected Penaeide was collected from shrimp farm at southern sea of Korea from 1993 to 1995. The Infectious virus was purified and used for diagnosis of infected shrimp. Anti-viral serum were used for immunological detection as enzyme linked immunoabsorbent assay(ELISA) and indirect fluorescent antibody technique(IFAT). In IFAT, stomach, lymphoid organ and antenae gland of infected shrimp showed fluorescent reaction. In ELISA, tissues of spontaneously infected shrimp appeared higher O.D. values than in artificial infected shrimp. Primer set was constructed from sequence of 420bp of cloned Baculovirus(WSBV) genome. Specific band for infected shrimp was detected in Polymerase chain reaction(PCR)

키워드

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