생명과학회지 (Journal of Life Science)

  • 제9권4호
  • /
  • Pages.414-422
  • /
  • 1999
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

Pseudomonas alcaligenes JCL-43이 생산하는 Carrageenase의 정제 및 특성

Purification and Characterization of Carrageenase from Pseudomonas alcaligenes JCL-43

  • 주동식 (강릉대학교 동해안해양생물자원연구센터) ;
  • 조순영 (강릉대학교 동해안해양생물자원연구센터) ;
  • 이정석 (부경대학교 식품공학과) ;
  • 이응호 (부경대학교 식품공학과) ;
  • 양승택 (경성대학교 식품공학과)
  • 발행 : 1999.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Our works performed for preparation of oligosaccharides from carrageenan, seaweed polysaccharide, and one active strain for carrageenan was isolated from sea water and identified to Pseudomonas alcaligenes. Carrageenan degrading enzyme was purified from the culture fluid of isolated strain-Pseudomonas alcaligenes JCL-43, by DEAE-Cellulose, Sephadex G-100, Q-Sepharose and CM Sepharose CL-6B column chromatography. Two enzyme-F-I, F-II- was identified this purifying process, and the molecular weight of the purified carrageenase were estimated to be 23.6kDa and 30.2kDa, respectively. The optimum pH and temperature for two carrageenase activity were 7.0 and 4$0^{\circ}C$. These enzymes were stable in the pH range of 6.0~7.5 and lower than 5$0^{\circ}C$, and required 1.5% NaCl for optimum activity. And these carragennase were inhibited by metal ions such as Cu2+, Zn2+, Hg2+, but increased by Ba2+ and Ca2+, and showed specificity on -carrageenan.

키워드

참고문헌

  1. Can. J. Microbiol v.5 An interpretation of the effects of salts on the lactic dehydrogenase of Halobacterium salinarium Baxter, R. M.
  2. Ann. New. York Acad. Sci. v.121 Disc-electrophoresis II. Method and application to human serum protein Davis, B. J.
  3. Carbohydrate and Carbohydrate Polymers De Clercp, E.;M. Yalpani(Ed)
  4. Kor. J. Biotechnol. Bioeng v.13 no.4 Preparation of agar hydrolysates by agarase and functionality of the hydrolysates Joo, D. S.;Cho, S. Y.;Lee, E. H.
  5. Kor. J. Appl. Microbiol. Bitechnol v.21 no.3 Isolation of alginate degrading bacteria and production of alginate degrading activities by bacteria Joo, D. S.;Choi, S. Y.;Lee, E. H.
  6. J. Kor. Soc. Food Nutr. v.22 no.2 Purification of extracellular enzyme produced by Vibrio sp. Joo, D. S.;Lee, E. H.
  7. Kor, J. Food Sci. Technol v.27 no.1 Changes in functional properties of alginic acid by enzynatic degradation Joo, D. S.;Lee, J. S.;Cho, S. Y.;Shin, S. J.;Lee, choE. H.
  8. Kor. J. Food Sci. Technol v.28 no.1 Preparation of oligosaccharides from alginic acid by enzymatic hydrolysis Joo, D. S.;Lee, J. S.;Park, J. J.;Cho, S. Y.;Kim, H. K.;Lee, E. H.
  9. Kor. J. Biotechnol. Bioeng v.13 no.1 Characterization and purification of agarase from Cytophaga sp. ACLJ-18 Joo, D. S.;Song, H. M.;Lee, J. S.;Cho, S. Y.;Lee, E. H.
  10. Nature v.227 Cleavage of structural proteins during the assmbly of the bacteriophage T4 Laemmli, U. K.
  11. J. Biol. Chem. v.193 Protein measurement with the folinphenol reagent Lowry, O. H.;N. J. Rosebrough;A. L. Farr;R. J. Randall
  12. Eur. J. Biochem v.201 Purification and characterization of a new Kappa-carrageenase from marine Cytophaga-like bacterium Potin, P.;A. Saneau;Y. Le Gall;C. Rochas;B. Kloareg
  13. Mucopolysaccharides of higher animals, The Carbohydrates, Chemistry and Biochemistry Roger, W. J.;W. Pigman;D. Horton
  14. J. Biol. Chem. v.195 Notes on Sugar Determination Somogyi, M.;N. Nelson
  15. Kor. J. Appl. Microbiol. Bitechnol v.24 no.6 Isolation and identification of carrageenan degrading bacteria and optimization of cnzyme production Yang, S. T.;Joo, D. S.;Park, J. J.;Lee, J. S.;Kim, M. S.;Lee, E. H.
  16. 化學と生物 v.30 微生物による天然多糖からの有用オリゴ糖の生産 富田旁男;構田 馬
  17. 海藻の本, 硏究士 西澤一俊;村杉幸子
  18. New Food Industry v.32 ケル化劑, 增粘劑としてのカラギ-ナンの特性とその商品ヘの應用例について 岩瀨弘土郞
  19. 日本農藝化學會誌 v.61 フラクトオリゴ糖の工業生産とその利用開發 日高秀昌;榮田利章;足立 曉;劑藤安弘
  20. 日本農藝化學會誌 v.67 糖鎖の生物機能 佐佐木隆造
  21. 식품과학과 산업 v.25 탄수화물 신조재의 개발 박관화
  22. 생물공학 News v.1 Carbohydrates/polysaccharides의 의학적 이용 및 현재의 개발 현황 박준태