생명과학회지 (Journal of Life Science)

  • 제9권4호
  • /
  • Pages.382-388
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  • 1999
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

간배양 HepG2 세포의 지질대사에 미치는 Hesperidin 및 Naringin의 영향

Effects of Citrus Flavonoid, Hesperidin and Naringin on Lipid Metabolism in HepG2 Cells

  • 김범규 (경상대학교 유전공학 연구소) ;
  • 차재영 (동아대학교 생명자원과학부) ;
  • 조영수 (동아대학교 생명자원과학부)
  • 발행 : 1999.08.01
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초록

The effects of citrus flavonoids, hesperidin and naringin, on the lipid metabolism were investigated in cultured human hepatocyte HePG2 cells. HepG2 cells were cultured for 6 h and 24 h to the control medium or the media containing hespridin and narigin, which concentrations were 0.5 and 5.0 mg/$m\ell$. There were no significant effects on cell proliferation and cellular protein content, except for increased in these parameters by adding both citrus flavonoids (0.5 mg/$m\ell$). The cellular content of triacylglycerol after 6 h incubation with 0.5 mg/$m\ell$ hesperidin and naringin was markedly increased, and after 24 h incubation that was decreased in both citrus flavonoids supplementation. The supplementation of 5.0 mg/$m\ell$ hesperidin caused a marked decrease in the cellular cholesterol content following 6 h incubation, and that was also reduced markdly, in a dose-dependent manner, during incubation for 24 h. However, there was no significant difference in the cellular cholesterol content in medium supplemented with naringin. The effect of hesperidin and naringin on acyl-CoA: cholesterol acyltransferase (ACAT) activity was studied in vivo and in vitro. The data confirmed that hesperidin inhibit ACAT activity in vivo and in vitro, whereas naringin had no such effect on ACAT activity in vivo but not in vitro. The present study suggests that hesperidin reduces the cellular triacyglycerol and cholesterol contents in human hepatocyte HepG2 cells.

키워드

참고문헌

  1. Clin. Pharmacol. Ther. v.57 Metabolism of naringin and hesperidin Ameer, B.;Weintraub. R.;Johnson, J.
  2. Am. J. Cardiol v.70 Relation of HDL cholesterol and triglycerides to incidence of atherosclerotic coronary artery disease (The PROCAM Experimence) Assmann, G.;Schulth, H.
  3. J. Exp. Biol. v.19 Cholesterol lowering action of vitamin P-like compounds in rats Basarkar, P. W.;Nath, N.
  4. Can. J. Biochem. Physiol. v.37 A rapid method of total lipid eatraction and purification Bligh, E. G.;Dyer, W. J.
  5. Recent Prog. Hormone Res. v.35 Receptor mediated uptake of lipoprotein cholesterol and its utilization for steroid synthesis in the adrenal cortex Brown, M. S.;Kovanen, P. T.;Goldstein, J. L.
  6. Agri. Chem. Biotech v.40 Effects of hesperidin, naringin and their aglycones on the in vitro assay phosphatidate phisphohydrolase, and on the proliferation in cultured human hepatocytes HepG2 ells Cha, J.-Y.;Cho, Y.-S.
  7. 51th annual Meeting of Japanese Society of Nutrition and Food Science Preventive effect of hesperetin on orotic acid-induced fatty liver Cha, J.-Y.;Mameda, Y.;Furukawa, J.;Rahaman, M.;Anno, N.;Yanagita, T.
  8. Biosci. Biotechnol. Biochem v.62 Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phosphatidate phosphohy-drolase activity in rats Cha, J.-Y.;Maeda, Y.;Oogami, K.;Yamamoto, K.;Yanagita, T.
  9. Free Radical Biol. Med. v.9 Flavonoids as superoxide scavegers and antioxidants Chen, Y. T.;Zheng, R. L.;Jia, Z. J.;Ju, J.
  10. J. Natl. Produc. v.54 Antihyperlipidemic effects of flavonoids from Prunus Davidiana Choi, J. S.;Yokozawa, T.;Oura, H.
  11. IL Farmaco. v.50 Biological effects of heperidin, a citrus flavonoid. (note II): Hypolipidemic activity on experimental hypercholesterolemia in rat Curto, R. B. L.
  12. Biometrics v.1 Multiple range and multiple Ftests Duncan, D. B.
  13. Lancet v.342 Dietary antioxidant fravonoids and risk of coronary heart disease the Zutphen Elderly Study Hertog, M. G. L.;Fesken, E. J. M.;Hollman, P. C. H.;Katan, M. B.;Kromhout, D.
  14. Arch. Intern. Med. v.155 Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study Hertog, M. G. L.;Kromhout, D.;Aravanis, C.;Blackburn, H.;Buzina, R.;Fidanza, F.;Giampaoli, S.;Jansen, A.;Menotti, A.;Nedeljkovic, S.;Pekkarinen, M.;Simic, B. S.;Toshima, H.;Fesken, E. J. M.;Hollman, P. C. H.;Katan, . B.
  15. Annual Regional Meeting of Agricultural Chemical Society of Japan Effect of dietary hesperidin and their aglycone on the lipid metabolism in rats Hisatomi, T.;Hara, E.;Furukawa, J.;Cha, J.-Y.;Anno, N.;Yanagita, T.
  16. Biocsi. Biotech. Biochem v.61 Hesperidine as an inhibitor of lipases from porcien pancreas and pseudomonas Kawaguchi, K.;Mizuno, T.;Aida, K.;Uchino, K.
  17. Br. Med. J. v.312 Flavonoid intake and coronary mortality in Finland: a cohort study Knekt, P.;Jarvinen, R.;Reunanen, A.;Maatela, J.
  18. J. Lipid Res. v.34 vivo evidence that lipidregulating activity of the ACAT inhibitor Ci-976 in rats is due to inhibition of both intestinal and liver ACAT Krause, B. R.;Anderson, M.;Bisgaier, C. L.;Bocan, T.;Bousley, S.;DeHart, S.;Essenburg, A.;Hamelehle, K.;Homan, R.;Kieft, K;Mcnally, W.;Stanfield, R.;Newton, R. S.
  19. World Rev. Nutr. Diet v.24 The Flavonoids. A class of semiessential food components: their role in human nutrition Kuhman, J.
  20. Biochem. Biophys. Acta v.348 Glycerolipid formation from sn-glycerol-3-phosphate by rat liver cell fractions Lamb, R. G.;Fallon, H. J.
  21. Biochem. Pham. v.42 Inhibition of mammalian 5-lipoxygenase and cyclo-oxigenase by flavonoids and phenolic dietary additives Laughton, M. J.;Evana, P. J.;Moroney, M. A.;Hoult, J. R. S.;Halliwell, B.
  22. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry, O. H.;Rosenbrough, N. J.;Farr, A. L.;Ranll, R. J.
  23. Circulation v.85 Triglycerides and LDL cholesterol and HDL cholesterol concentration on coronary heart disease cholesterol concentration on coronary heart disease risk in the Helsinki Heart Study Manninen, V.;Tenkanen, L.;Koskinen, P.;Huttunen, J. K.;Manntari, M.;Heinonen, O. P.;Frick, M. H.
  24. J. Agric. Food. Chem. v.42 Differentiation of citrus juices by factorial discriminant analysis using liquid chromatography of flavanone glycosides Mouly, P. P. M.;Arzouyan, C. G.;Gaydou, E. M.;Estienne, J. M.
  25. Biosci. Biotechnol. Biochem. v.56 A modified colorimetric MTT assay adapted for primary cultured hepatocytes: Application to proliferation and cytotoxity assays Oka, K.;Maeda, S.;Koga, N.;Kato, K;Saito, T.
  26. FEBS Lett. v.401 Pagana, G.;Rice-Evans, C. A.
  27. Acta Vitaminol. Enzymol. Enzymol v.5 Action of vitamin P like components on lysosomal status in hypercholesterolemic rats Rathi, A. B.;Nath, N.;Chari, S. N.
  28. Lancet v.339 Wine, alcohol, Phatelets, and the French paradox for coronary heart disease Renand, S.;de Lorgeril, M.
  29. J. Agric. Food. Chem. v.35 Quantitative survey of narirutin, naringin, hesperidin and neohesperidin in citrus Rousff, R. L.;Martin, S. F.;Youtsey, C. O.
  30. 49th annual Meeting of Japanese Society of Nutrition and Food Science The effect of naringin and it aglycon on the lipid metabolismes in rats Yanagita, T.;Hisatomi, T.;Nunez, J. F.;Cha, J.-Y.;Anno, N.
  31. Cur. Therapeutic Res. v.56 Effect of a new acly-coenzyme A : cholesterol aclytransferase inhibitor, HL-004, on cholesterol esterification and lipid metabolism in HepG2 cells Yanagita, T.;Sonda, K.;Yamamoto, K.;Yotsumoto, H.;Nunez, H.J.;Murakami, S.
  32. Cli. Therapeutics v.16 Effect of simvastatin, a cholesterol synthesis inhibitor, on phosphatidylcholine synthesis in HepG2 cells Yanagita, T.;Yamamoto, K.;Ishida, S.;Sonda, K.;Morito, F.;Saku, K.;Sakai, T.
  33. Planta Med. v.63 Inhibitory effect of Oren-Gedoku-to and its components on cholesteryl ester synthesis in cultured human hepatocyte HepG2 cells : Evidence from the cultured HepG2 cells and in vitro assay of ACAT Yotsumoto, H.;Yanagita, T.;Yamamoto, K.;Ogawa, Y.;Cha, J.-Y.;Mori, .
  34. Bull. Fac. Agr. Saga Univ. v.81 Effects of cis, cis-octade-cadienoic acid and serum albumin on lipid metabolism in HepG2 cells Yotsumoto, H.;Hara, E.;Hu, Y.;Nunez, J. F.;Cha, J.-Y.;Yanagita, t.
  35. Methods Biochem. Anal. v.21 Techniques for the characterization fo UDP-glucuronyltransferase, glucose-6-phosphate, and other tightly bound microsomal enzymes Zakim, D.;Donald, V. A.