생명과학회지 (Journal of Life Science)

  • 제9권4호
  • /
  • Pages.341-347
  • /
  • 1999
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

멧누에(Bombyx mandarina)로부터 Chitinase를 코딩하는 cDNA의 분리 및 염기서열 결정

Molecular Cloning and Characterization of the Gene Encoding Chitinase from Bombyx mandarina

  • 구태원 (농촌진흥청 농업과학기술원) ;
  • 황재삼 (농촌진흥청 농업과학기술원) ;
  • 성규병 (농촌진흥청 농업과학기술원) ;
  • 윤은영 (농촌진흥청 농업과학기술원) ;
  • 방혜선 (농촌진흥청 농업과학기술원) ;
  • 권오유 (충남대학교 의과대학)
  • 발행 : 1999.08.01
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초록

Insects use chitinolytic enzyme to digest chitin in the exoskelton during the molting process. We have isolated and sequenced a chitinase-encoding cDNA from the silkworm, Bombyx mandarina, compared its sequenced with genes encoding chitinolytic enzymes from other sources. The insert DNA in the clone is 2,675 nucleotides long with an open reading frame of 1,695 uncletides that encodes a protein of 565 amino acids with a molecuar weight of 63.4 kDa. The 3' -untranslated region of 889 nucleotides is AT-rich and contains two putative polyadenylation signals. The N-terminal sequence of the encoded protein contains numerous hydrophobic residues characteristic of a leader peptide. The amino acid alignment revealed that the endo-$\beta$-N-acetylglucosaminidase had 83% and 97% homology with M. sexta and B. mori, respectively. The deduced amino acid had two highly conserved region at the amino acid residues 97-111 and 139-148 that were related to the existing chitinase.

키워드

참고문헌

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