The Stimulatory Effect of $Ca^{2+}and; Mg{^2}+ $ lons on the Formation of Protein Aggregate during in vitro Refolding of Tryptophan Synthase $\alpha$-Subunit

트립토판 중합효소 알파 소단위체의 in vitro 구조재형성시 $Ca^{2+}과; Mg^{2+} $ 이온의 단백질 응집체형성 촉진 효과

The effect of cations on the formation of protein aggregates was examined by in vitro refolding of mutant tryptophan synthase $\alpha$-subunit in which Pro 24 was replaced by Leu. $NH^{4+},; K{^+}; and; Na^{+}$ and no effect, but $Mg^{2+}; and; Ca^{2+}$stimulated the formation of protein aggregates in dose-dependent manner. It is suggested that $Mg^{2+} and Ca^{2+}$ may be implicated in the formation of protein aggregates in vivo.