Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characteristics of Immobilized Rhodopseudomonas sp. for Wastewater Treatment

폐수처리를 위한 고정화 Rhodopseudomonas sp.균의 특성

  • 이범규 (부경대학교 식품생명공학부 생물공학전공) ;
  • 김상희 (부경대학교 식품생명공학부 생물공학전공) ;
  • 김중균 (부경대학교 식품생명공학부 생물공학전공)
  • Published : 1999.06.01
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Abstract

Rhodopseudomonas sp. was immobilized in three supports(agar, k-carrageenan, and PVA) in order to remove nitrate in wastewater coming from fish farm. Among them 3% agar was the most suitable support when denitrification rate and bead durability were tested. Optimum bead size was 4mm-diameter when the substrate transfer into the bead and shear stress for bead were considered, and optimum cell loading was 25mg dry $cells/cm^2$gel gel. Ethanol was the best as a carbon source, and optimum C:N ratio, temperature and pH were 1.5:1, $31^{\circ}C$,, and 6, respectively. Under these conditions the maximum denitrification rate in synthetic wastewater was $$345{\MU}{\ell};N_2/Cm^3 gel{\cdot}hr;and that in modified MYC medium was 450{\MU}{\ell}};N_2/Cm^3 gel{\cdot}hr $$.

고저화된 Rp.palustris균을 이용한 양식장 폐수주으이 질산염 제거를 위하여 Rp.palustris균의 고정화를 위한 담체를 조사하였는데, 탈질능력과 내구성을 실험한 결과 agar,k-carrageenan,그리고 PVA 3종의 담체주우 3% agar가 가장 적합한 담체이었다.Agar bead 내부로의 기질 이동 및 sheer stress를 고려해볼 때 최적 bead 크기는 직경 4mm 였고,접종되는 세포의 양은 25mg dry $cells/cm^2$gel 이었다. 탄소원으로 ethanol이 가장 적합하였고,최적 C:N ratio는 1.5이며,온도와 pH는 각각$31^{\circ}C$,PH 6 이었다. 이러한 조건에서의 최대 탈질율은 인공합성폐수의 경우 $345{\MU}{\ell};N_2/Cm^3 gel{\cdot}hr;이었으며,;modifed MYC 배지의; 경우는; 450{\MU}{\ell}};N_2/Cm^3 gel{\cdot}hr $이었다.

Keywords

References

  1. Appl. Environ. Microbiol. v.32 no.6 Nitrate removal in closed-system aquaculture by columnar denitrification Balderston, W. L.;J. M. Sieburth
  2. Am. J. Clin. Nutr. v.25 Commentary on the Hungate's technique for culture of anaerobic bacteria Brynet, M. N.
  3. J. Environ, Syst. v.17 Treatment alternatives for nitrate contaminated groundwater supplies Dahab, M. F.
  4. J. Wat. Pollut. Control Fed. v.60 Nitrate removal from water supplies using biological denitrification Dahab, M. F.;Y. W. Lee
  5. Biotechnol. Bioeng. v.56 no.5 Relevance of rheological properties of gel beads for their mechanical stability in bioreators dos Santos;V. A. M.;E. J. T. M. Leeneu;M. M. Rippoll;C. van der Sluis;T. van Vliet;J. Tramper
  6. Biotechnol. Lett. v.6 Hydrogen production by Rhodopseudomonas capsulata cells entrapped in carrageenan beads Franco, N.;P. M. Vagnais
  7. Appl. Microbiol. Biotechnol. v.48 Nitrate removal from drinking water using a membarne-fixed biofilm reactor Fuchs, W.;R. Schatzmayr;R. Braum
  8. J. Envir. Engng. v.115 Biological denitrification of water Gayle, B. P.;G. D. Boardman;J. H. Sherrard;R. E. Benoit
  9. Biotechnol. Bioeng. v.30 Immobilization of activated sludge by PVA boric acid method Hashimoto, S.;K. Furukawa
  10. Argric. Biol. Chem. v.50 no.4 Photoproduction of hydrogen by immpbilized cells of a photosynthetic bacterium, Rhpdospirilllum rubrum G-9 BM. Hirayama, O.;K. Uya;Y. Hiramatsu;H. Yamada;K. Moriwaki
  11. The photosynthetic bacteria Hoshino, Y.;H. Kitamura;Seitai;Kitamura, H.(ed.);Morita, S.(ed.);Yamashita, J.(ed.)
  12. Appl. Biochem. Biotechnol. v.50 no.3 Screening of a growing cell immobilization procedure for the biosynthesis of thermostable α-amaylase Ivanova, V.;M. Stefanova;A. Tonkova;E. Dovera;D. Spassova
  13. Fish and River Pollution Jones, J. R. E.
  14. Aquacult. Eng. v.19 Charaterization of denitrifying photosynthetic bacteria isolated from photosynthetic sludge Kim, J. K.;B. K. Lee;S. H. Kim;J. H. Moon
  15. J. Korean Fish. Soc. v.30 no.5 Immobilization of consortium for the removal of ammonium ion in the recirculating aquaculture system Kim, S. K.;J. K. Seo;J. S. Lee;I. S. Kong;K. H. Suh
  16. FEMS Microbiol. Lett. v.9 Dissimilatory nitrate reduction by strains of the acultative phototrophic bacterium Rhodopseudomonas palustris klemme, J. H.;I. Chyla;M. Preuss
  17. J. Ferment. Technol. v.49 Construction of a purification plant for polluted water using photosynthetic bacteria Kobayashi, M.;M. Kobayashi;H. Nakanishi
  18. The nitrogen and sulfur cycles Ecology of nitrification and denitrification Kuenen, J. G.;L. A. Robertson;J. A. Cole(ed.);S. J. Ferguson(ed.)
  19. Wat. Res. v.29 no.1 Denitrification and methanogenesis in a co-immobillized mixed culture system Lin, Y. F.;K. C. Chen
  20. Prog. Water Technol. v.8 N-nitroso Compounds, Nitrate, and nitrite: Possible implications for the Causation of Human Cancer Mirvish, S. S.
  21. Eur. J. Appl. Microbiol. Biotechnol. v.14 Columnar denitrification of water by immobilized pseudomonas denitrificans cells Nilsson, I.;S. Ohlson
  22. Eur. J. Appl. Microbiol. Biotechnol. v.10 Denitrification of water using immobilized pseudomonas dentrificans cells Nilsson, I.;S. Ohlson;L. Haggstrom;N. Molin;K. Mosbach
  23. The photosynthetic bacteria General physiology and ecology of photosynthetic bacteria;Clayton, R. K.(ed.);Sistrom, W. R.(ed.) Pfennig, N.
  24. Water Res. v.6 Microbial mitrification and denitrification in concentrated waters Prakasam, T. B. S.;R. C. Loehr
  25. J. Ferment. Bioeng. v.72 Hydrogen production and denification by photosynthetic bacteria isolated from lake Nakaumi and its vicinity Shen, J.;O.Hirayama
  26. Water Technology. v.4 Pilot plant research on treatment of citric acid fermantation water by a photosynthetic bacteria method Shen, J.;J. Yu.;Y. Gu.;S. GE.;Z. Zhu.;Y. Huo.
  27. Biotechnol. Bioengin. v.17 Single-cell protein production by photosynthetic bacteria cultivation in agricultural by-products Shipman, R. H.;I. C. Kao.;L. T. Fan.
  28. Prog. Water Technol. v.12 Infant Methemoglobinemia and other Health Effects or nitrates in drinking water Shuval, H. I.
  29. J. Am. Water Works Assoc. v.70 Treatment technology to meet the interim primary drinking water regulation for inorganics Sorg, T. J.
  30. J. Am. Water Works Assoc. v.61 Treatment of high nitrate waters St. Amant, P. P.;P. L. McCarty
  31. Annu. Rev. Microbiol. v.43 Biochemistry of acetate metabolism in anaerobic chemotrophic bacteria Thauer, R. K.;D. Moller-Zinkhan;A. M. Spormann
  32. Aquaculture v.133 Anaerobic treatment of intensive fish culture effluents: digestion of fish feed and release of volatile fatty acids van Rijn, J.;N. Fnarev;B. Berkowitz
  33. Int. J. Hydrogen Energy v.7 Hydrogenproduction by immobilized cell. Ⅱ. H₂-photoevolution and wastewater treatment by agar-entrapped cells of Rhodopseudomonas palustris and Rhodospirillum molischianum Vincenzini, M.;R. Materassi;M. R. Tredici;G. Florenzano
  34. Int. J. Hydrogen Energy v.7 Hydrogen production by immobilized cell. Ⅰ. Light dependent dissimilation of organic substances by Rhodopseudomonas palustris Vincenzini, M.;R. Materassi;M. R. Tredici;G. Florenzano
  35. Am. Soc. Agric. Eng. Biological dentrification in closed recirculating marine culture system. Technique for Modern Aquaculture Whitson, J.;P. Turk;Wang, J. K.(Ed.)
  36. Water Works Assoc. v.63 Nitrate in drinking water Winton, E. F.