Screening of Transcriptional Regulator of the Draf Proto-oncogene Using the Yeast One-hybrid System

  • Park, So-Young (Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735) ;
  • Park, Na-Hyun (Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735) ;
  • Kwon, Eun-Jeong (Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735) ;
  • Yoo, Mi-Ye (Corresponding author, Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735)
  • Published : 1999.10.01

Abstract

The Raf, a cytoplasmic serine/thereonine protein kinase, acts as an important mediator of signals involving cell proliferation, differentiation and development. Multiple regulatory elements should participate in the expression of D-raf, Drosophila homolog of human c-raf-1. In order to search regulatory factors involved in the D-raf promoter activation, we accomplished the yeast one-hybrid screening using D-raf promoter region from bp-330 to -309 with respect to the transcription initiation site as bait. After screening, sixteen independent positive clones of ${\beta}$-galactosidase activties were identified and sequenced. Two clones having 94-98% identity with daughterless and one clone having 93% identity with escargot by Blast search among these clones were screened.

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