소양호 용존 유기물의 분자량 크기에 따른 세균수와 $\beta$-Glucosidase 활성도의 변화

The Change of Bacterial Numbers and $\beta$-Glucosidase Activities by the Size Fraction of DOM in Lake Soyang

  • 김굉규 (강원대 환경학과 및 서울대 분자미생물학 연구센터) ;
  • 안태석 (강원대 환경학과 및 서울대 분자미생물학 연구센터) ;
  • 김동주 (강원대 환경학과 및 서울대 분자미생물학 연구센터) ;
  • 홍선희 (강원대 환경학과 및 서울대 분자미생물학 연구센터) ;
  • 최승익 (서남대 환경 보건학과)
  • 발행 : 1999.03.01

초록

소양호에서 식물 플랑크톤이 분비하는 유기물의 분자량 크이게 따른 세균수와 활성의 변화를 측정하였다. 소양호 상걸리 유역에서 여름철에 채수한 물을 tangential flow ultrafiltration 으로 용존 유기물질을 100,000 nMW~0.1$\mu\textrm{m}$, 10,000 nMW~100,000 nMW와 1,000 nMW~10000 nMW의 3개 fraction으로 구분하였고, 여기에 호숫물을 접종하여, 세균수와 $\beta$-glucosidase 의 변화를 측정하였다. 배양기간 동안에 나타난 총세균수는 24시간까지 급격히 증가한 후 점차 안정적으로 변하는 전형적인 성장곡선을 나타내었으며, DOC 농도와 종류가 달랐음에도 , $1.2{\times}10^{7}$ cells $ml^(-1)$범위였고, 저분자 fraction에 비해 최고 1,000배 이상 높았다. 즉 10,000 nMW 이상의 고분자 용존 유기물질은 $\beta$-glucosidase의 유도체로 작용하며, 저분자 용존 유기물질은 $\beta$-glucosidase 활성도를 높이지 못하는 것으로 확인되었다.

Effects of different molecular size fractions (100,000 nMW-0.1 $\mu\textrm{m}$m, 10,000 nMW-100,000 nMW and 1,000 nMW-'L0,000 nMW) of dissolved organic matter on the bacterial numbers and $\beta$-glucosidase activities in Lake Soyang were investigated. Even though the concentrations and characteristics of each fractions were different, bacterial growth curves of each fractioii were Lypical and similar. Each growth curve had highest peak of $1.1{\times}10^{7}$ cells $ml^{-1}$. But, the $\beta$-lucosidase activitics of each fraction were quite different. In high molecular weight fraction (HMW: 100,000 nMW-0.1 $\mu\textrm{m}$m), Vmax of Pglucosidase activity ranged from 550 to 1,160 nmol $1^{-1}$.$hi^{-1}$, but in low molecular weight f~action (1,000 nMW-10,000 nMW), that ranged from 1 to 14 om01 1$^[-1}$${-1}$

키워드

참고문헌

  1. 한국미생물학회지 v.32 소양호 지류에서 식물플랑크톤 변화에 따른 미생물 활성도의 일변화 김상종;안태석;안태영;조기성;곽노태;이동훈;박호완
  2. 한국미생물학회지 v.33 호수 생태계의 생화학적 분석 최승익;안태석
  3. 소양호 세균개체수와 활성도 변화에 관한 연구 최승익
  4. Verh. Internat. Verein. Limnol. v.26 The different fractions of organic matter and its annnual changes in a eutrophied Brazilian coastal lagoon Bernardes, M. C.;K. N. Kuroshima;J. P. Carmouze
  5. Limnol. Oceanogr. v.34 Characterization and significance of β-glucosidase activity in lake water Chrost, R. J.
  6. J. Plankton Res. v.11 Photosynthetic production and exoenzymatic degradation of organic matter in the euphotic zone of eutrophic lake Chrost, R. J.;U. Munster;H. Rai;D. Albercht;P.K. Witzel;J. Overbeck
  7. Appl. Environ. Microbiol. v.33 Use of Nucleopore filters for counting bacteria by fluorescence microscopy Hobbie, J. E.;R. J. Daley;S. Japer
  8. Mar. Eco. v.11 Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl-substrate Hoppe, H. G.
  9. Limnol. Oceanogr. v.34 The measurement and distribution of dissolved nucleic acids in aquatic environments Karl, D. M.;M.D. Bailiff
  10. Microb. Ecol. v.13 Bacterial growth in dissolved organic matter from a blackwater river Mayer, J. L.;R. T. Edwards;R. Risley
  11. Appl. Environ. Microbiol. v.63 Phosphorus and bacterial growth in drinking water Miettinen, I. T.;T. Vartiainen;P. J. Martikainen
  12. Biochemical and Molecular Approaches Origin, composition and microbial utilization of dissolved organic matter. In Aquatic Microbial Ecology Munster, U.;R. J. Chrost;Overback, J.;Chr st, R.J.(eds.)
  13. Microbial enzymes in Aquatic Environments Extracellular enzyme activity in eutrophic and polyhumic lakes Munster, U.;Chrost(ed.)
  14. Microbial ecology of Lake Pluβsee Dissolved organic matter: Analysis of composition and function by a molecular-biochemical approach Munster, U.;D. Albrecht.;J. Overbeck;R.J. Chrost(eds.)
  15. Appl. Envrion. Microbiol. v.56 Production of dissolved DNA, RNA, and protein by microbial populations in a Florida reservoir Paul, J. H.;W. H. Jeffrey;J. P. Cannon
  16. Aquatic microbiology(2nd ed.) Rheinheimer, G.
  17. Microbial ecology of Lake Pluβsee Phytoplankton-excreted organic carbon Sell, A. F.;Overback; R.J. Chrost(eds.)
  18. Nature v.347 Infection of phytoplankton by viruses and reduction of primary productivity Suttel, C.A.;A. M. Chan;M. T. Cottrell
  19. Mar. Ecol. Prog. Ser. v.23 Interactions of bactivorous grazers and heterotrophic bacteria with dissolved orgnic matter Taylor, G. T.;R. Iturriaga;C. W. Sullivan
  20. Hydrobiologia v.229 Effect of different molecular weight fractions of dissolved organic matter on the growth of bacteria, algae and protozoa from a highly humic lake Tulonen, T.;K. Salonene;L. Arvola