Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Biochemical and Cytological Changes of Pseudomonas sp. DJ-12 Cells in Response to Catechol Treatment

Catechol 처리에 의한 Pseudomonas sp. DJ-12의 생화학 및 세포학적 변화

  • 고연자 (충북대 자연과학대학 미생물학과) ;
  • 임재윤 (충북대 자연과학대학 미생물학과) ;
  • 김치경 (충북대 자연과학대학 미생물학과) ;
  • 이기성 (배재대 자연과학대학 생물학과)
  • Published : 1999.06.01
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Abstract

Aromatic hydrocarbons which are not easily degraded by microorganisms can be accumulated in the conlaminated environment for a long lime, producing toxic effects on wild lives and humans. However, the sublethal concentrations of the chemicals induce the synthesis of stress-shock proteins in the cells and increase the adaptability of the organisms to the environmental stresses. In this study, therefore, the cells of Psezido~nonus sp. DJ- 12 treated with catechol at various concentrations were inveshgated for their survival, biodegtadability of catechol, production of stress-shock proteins, and cytological changes. The organisms were capable of degrading catechol at the range of 0.5 to 1.0 mM concentration wilhin 6 hours incubation, but they were killed by $10^2$-10$^3$ celllinl at 3 mM or higel- concentration without any catechol degradation. These cells treated with catechol begm lo produce DnaK and GroEL at 1 mM and 0.5 mM. respectively. Pseudumonas sp. DJ-12 treated with 10 mM catechol for I hour exhihiled some punctuated pores on the cell wall and contortion of the rod shape. The cells treated with he sublethal concentration of catechol showed the increased tolerance for suvival when exposed to the lethal concentration, and such tolerant effects were functioned crossly among benzoate, 4-chlorobenzoate, 'and catechol.

방향족 탄화수소 화학물질들은 자연계에 오염되면 미생물에 의한 분해가 미미하여 장기 축적됨으로써 생명체에 독성을 나타낸다. 이러한 방향족 탄화수소가 준치사 수준의 농도로 미생물에 노출되면 stress-shock 단백질을 합성하거나 세포 구성물질에 생화학적 변화가 일어나 적응현상이 나타나게 된다. 본 연구에서는 Pseudomonas sp. DJ-12를 여러 가지 농도의 catechol 로 처리했을 때 나타나는 stress-shock 단백질의 합성양상과 함께 세포의 형태와 생존을 위한 내성의 변화를 연구하였다. Pseudomonas sp. DJ-12는 0.5~1mM 의 catechol 이 6시간 배양 후 60% 이상이 분해되었으나, 3mM 도는 그 이상의 농도에서는 전혀 분해되지 않앗고 생존 세포수는 30시간 처리 했을 때부터 \10^2$ cell/ml 또는 그 이상이 사멸되었다. DnaK는 1mM 이상의 catechol 로 10분간 처리할 때 유도 생성되었고, GroEL은 0.5 mM 이상에서 생성되었다. 10mM 의 catechol로 처리한 Pseudomonas sp. DJ-12 의 세포는 세포벽에 구멍이 생겼으며 간균의 형태가 일그러지는 변화가 관찰되었다. 준치사 농도인 1mM의 catechol 이나 benzoate 또는 4-chlorobenzoate 로 전처리한 Pseudomonas sp. DJ-12는 stress-shock 단백질이 합성되었을 뿐 아니라, 치사 농도인 10mM 의 catechol에서 tolerance를 나타내었다.

Keywords

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