미생물학회지 (Korean Journal of Microbiology)

  • 제35권2호
  • /
  • Pages.107-114
  • /
  • 1999
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Fusarium 종에서의 RAPD-PCR분석

RAPD-PCR Analysis in Fusarium species

  • 발행 : 1999.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Fusarium 균에 속하는 16종 21균주를 대상으로 RAPD-PCR 방법을 이용하여 DNA 다형성을 분석하여 계통 유전학적 유연관계를 검토하였다. 40개의 random primer 로 시험하여 실험한 모든 종에서 다형성을 나타내는 11개의 primer를 선별하였다. RAPD 분석결과 평균 23.9개씩 모두 263개의 크기가 다른 RAPD 밴드들을 조사할 수 있었다. 각 primer에 대해 각각 독특한 DNA 다형성을 나타내었고, 증폭된 DNA 크기는 0.1-3.0 kb 범위에서 형성되었다. 각 균주간의 genetic similarity를 계산하여 유연관계를 dendrogram 으로 나타내었다. Genetic similarity 0.627을 기준으로 하여 크게 4그룹으로 나눌 수 있었다.

To assess genetic diversity amoug 21 strains from sixleen Frrsn~i~nn species , we used RAPD(rando1n amplified pol.ymorphic DNA) analysis based on PCR(po1ymerase chain reaction). Eleven primers showing Ule polymorphism were chosen from the 40 random pnmers-tcstcd. A total of 263 polymorphic bands were generated by the primers and the size of amplified DNA fragments ranged from 0.1 lo 3.0 kb. Sirnilku-it), coefficients between strains were calcnlatcd, and UPGMA cluster analysis was used to generate a dendrogram showing relationships among them. The results from RAPD-PCR analysis were grouped into four main groups at the si~nilarity level of 0.627.

키워드

참고문헌

  1. Plant Disease v.80 Use of RAPD markers as a diagnostic tool for the identification of Fusarium solani isolates that cause Soybean sudden death syndrome Achenbach, L. A.;J. Patrick
  2. Taxon v.42 Systematic relationships in Juniperus based on random amplified polymorphic DNA Adams, R. P.;T. Demeke
  3. Phytopathology v.84 Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by random amplified polymorphic DNA(RAPD) analysis Assigbetse, K. B.;D. Fernandez;M. P. Dubois;J. P. Geigern
  4. Mycol. Res. v.99 Genetic variation among 3 world-wide collection of isolates of Fusarium oxysporum f. sp. cubense analysed by RAPD-PCR fingerprinting Bentley, S.;K.G. Pegg;J.L. Dale
  5. Crypto-coccus neoformans. Curr. Genet. v.32 Variability of karyotypes and RAPD types in genetically related strains Boekhout, T.;A. van Belkum
  6. Can. J. Bot. v.65 An evalution of Fusarium oxysporum from crucifers based on pathogenicity, isozyme polymorphism, vegetative compatibility and geographical origin Bosland, B. W.;P. H. Williams
  7. Genome. v.38 Analysis of annual Medicago species using RAPD markers Brummer, E. C.;J. H. Bouton;G. Kochert
  8. Curr. Genet. v.20 Differentiation of Fusarium Solani f. sp. cucurbitae race 1 and 2 by random amplification of polymorphic DNA Crowhurst, R. N.;B.T. Hawthorne;E.H.A. Rikkerink ;M. D. Templeton
  9. Penicillium roqueforti. Curr. Cenet. v.24 Randomly amplified polymorphic DNAs assess recombination following an induced parasexual cycle Durand, N.;P. Reymond;M. Fevre
  10. Mycologia v.88 Morphological and RAPD analyses of Diaporthe phaseolorum from soybean Fernandez, F. A.;R.T. Hanlin
  11. Can. J. Bot. v.75 Grouping and identification of Tuber species using RAPD markers Gandeboeuf, D.;C. Dupre';P. Roeckel-Drevet;P. Nicolas;G. Chevalier
  12. A pictorial atlas. Mitt. Biol. Bundesanst. Land. Forstw. v.209 The Genus Fusarium Gerlach, W.;H. Nirenberg
  13. pisi. Phytopathol v.83 Use of random amplified polymorphic DNA(RAPD) to characterize race of Fusarium oxysporum f. sp. Grajal-Martin, M. J.;C. J. Simon;F. J. Muehlbauer
  14. PCR technology Current Innovations Griffin, H. G.;A. M. Griffin
  15. Phytopathology v.82 Random amplified polymorphic DNA markers: a system for identifying and differentiating isolates of Colletotrichum graminicola. Guthrie,P.A.I.;C.W.Magill;R.A. Fredericksen;G.N.Odvody
  16. Theoretical and Applied Genetics v.87 Comparison of RAPD and isozyme analyses of Avena sterilis L. accessions. Heun, M.;J. P. Murphy;T. D. Phillips
  17. Pant Pathol. v.46 Genetic diversity of Fusarium moniliforme detected by vegetative compatibility groups and random amplified polymorphic DNA markers Huang, R.;M. Galperin;Y. Levy;R. Perl-Treves
  18. A Guide to Method and Applications PCR Protocols Innis, M. A.;D. H. Gelfand;J. J. Sninsky;T. J. White
  19. Phytopathology v.81 Vegetative compatibility groups and subgroups in Fusarium oxysporum f. sp. radicis-lycopersici. Katan, T.;D. Zamir;M. Sarfatti;J. Kantan
  20. Phatopathology v.84 Use of genetic fingerprinting and random amplified polymorphic DNA to characterize pathotypes of Fusarium oxysporum f.sp. ciceris infecting chichkpea. Kelly, A.;A. R. Alcala'-Jime'nez;B. W. Bainbridge;J.B. Heale;I. Pe'rez-Arte's;R. M. Jime'nez-Diaz
  21. Phytopathol. v.82 Restriction fragment length polymorphism groups and physical map of mitochondrial DNA from Fusarium oxysporum f. sp. niveum. Kim, D. H.;R.D.Martyn;C. W. Magill
  22. Handbook of applied mycology:vol. 4. Fungal Biotechnology Molecular methods for identification and taxonomy of filamentous fungi Klich, M. A.;E. J. Mullaney;Dilip, K. A.;R. P. Elander;K. G. Mukerji(eds.)
  23. Curr. Genet. v.28 High-GC primers are useful in RAPD analysis of fungi Kubelik, A. R.;L. J. Szabo
  24. Gene Manipulation on Fungi Molecular taxonomy of the fungi Kurtzman, C.P.;Bennett J. W.;L. L. Lasure(eds.)
  25. Phytopathol. v.84 Use of the RAPD technique for identification of Fusarium oxysporum f. sp. dianthi from carnation Manulis, S.;N. Kogan;M. Reuven;Y. Ben-Yephet
  26. Rosa. Theor. Appl. Genet. v.92 Using RAPDs to study phylogenetic relationship Millan, T.;F. Osuna;S. Cobos;A. M. Torres;J. I. Cobero
  27. Kor. J. Mycol. v.26 Electrophoretic karyotyping by PFGE in the genus Fusarium Min, B. R.;J. S. Jung;Y. K. Choi
  28. PCR Methods Appl. v.1 The random amplification of polymorphic DNA for fingerprinting plants Munthali, M.;B. V. Ford-Lloyd;H. J. Newbury
  29. Applied and Environmental Microbiol. v.60 Characterization of the formae speciales of Fusarium oxysporum causing wilts of cucurbits by DNA fingerprinting with nuclear repetitive DNA sequences Namiki, F.;T. Shiomi;T. Kayamura;T. Tsugue
  30. Proc. Natl. Acad. Sci. USA v.76 Mathematical model for studying genetic variation in terms of restriction endonucleases Nei, M.;W. H. Li
  31. Peudocercosporella herpotrichoides. Mycol. Res. v.98 The use of random amplified polymorphic DNA to identify pathotype and direct variation Nicolson, P.;H. N. Rezanoor
  32. System. Appl. Microbiol. v.19 RAPD genomic fingerprinting differentiates Thiobacillus ferrooxidans strains. Novo, M.T.M.;A.P. Souza;G. J. Oswaldo;L. M.M. Ottoboni
  33. Phytopathology v.83 Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification Quellet, T.;K.A. Seifert
  34. Modern Assays for Plants Pathogenic Fungi ; Identification, Detection and Quantification RAPDs of Fusarium culmorum and F. graminearum for genotyping and identification Schilling, A. G.;E. M. Moller;H. H. Geiger;Schots, A.;F. M. Dewey;R. Oliver(eds.)
  35. Theor. Appl. Genet. v.86 High output genetic mapping of polyploids using PCR-generated markers Sobral, B.W.S.;R.J. Honeycutt.
  36. Can. J. Plant. Pathol. v.4 Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum Sutton, J. C.
  37. Phytopathology v.86 Characterization of single clonal lineage of Fusarium oxysporum f. sp. albedinis causing Bayoud disease of date palam in Morocco Tantaoui, A.;M. Quinten;J. P. Geiger;D. Fernandez
  38. Mycoscience v.37 Identification of the DNAs of the ectomycorhizal fungus Tricholoma bakamatsutake using specific oligonucleotide probes and PCR primers Terashima, Y.;T. Nakai
  39. Molecular systematics Nucleic acid Ⅱ: restriction site analysis Thomas, E. D.;C. Moritz;J. D. Palmer;David, M. H.;C. Moritz(eds.)
  40. Sydowia v.48 no.1 Ribosomal DNA analyses challenge the status of Fusarium sections Liseola and Elegans Waalwijk, C.;R.P. Baayen;J.R.A. de Koning;W. Gams
  41. DNA fingerprinting in plants and fungi Weising, K. H.;Nybom;K. Wolff;W. Meyer
  42. Nucl. Acids Res. v.18 Fingerprinting genomes using PCR with arbitrary primers Welsh, J.;M. McClelland
  43. Nucleic Acids Rese. v.18 DNA polymorphisms amplified by arbitrary primers are useful as genetic as genetic markers Williams, J.G.K.;A.R.Kubelik;K.J.Livak;J.A. Rafalski;S. V. Tingey
  44. Methods Enzymol. v.218 Genetic analysis using random amplified polymorphic markers Williams, J.G.K.;M.K. Hanafey;J.A. Rafalski;S. V. Tingey
  45. Phytopathology Current status of Fusarium taxonomy Windels, C. E.
  46. Phytopathology v.86 Characterization of Fusarium oxysporum f. sp. phaseoli by pathogenic races, VCGs, RFLPs, and RAPD Woo, S. L.;A. Zoina;G. Del Sorbo;M. Lorito;B. Nanni;F. Scala;C. Noviello
  47. Plants Pathol. v.45 Isozyme and RAPD-PCR analyses of Fusarium avenaceum strains from Finland Yli-Mattila;T. S. Paavanen;A. Hannukkala;P. Parikka;R. Tahvonen;R. Karjalainen