Internal Transcribed Spacer와 5.8S ribosomal DNA의 염기서열 분석에 의한 Agaricus blazei와 근연종에 대한 계통분류학적인 연구

Phylogenetic Analysis of Agaricus blazei and Related Taxa by Comparing the Sequences of Internal Transcribed Spacers and 5.8S rDNA

  • 김기영 (부산대 자연과학대학 미생물학과) ;
  • 하명규 (기초과학지원연구소, 부산분) ;
  • 이태호 (부산대 자연과학대학 미생물학과) ;
  • 이재동 (부산대 자연과학대학 미생물학과)
  • 발행 : 1999.09.01

초록

Agaricus 속의 계통분류학적 유연관계를 알아보기 위하여 Internal transcribed spacers 1,2, 와 5.8S rDNA 에 해당하는 부위를 PCR 기법으로 증폭하여 염기서열을 비교 조사하였다. 본 실험에 사용되어진 aGARICUS 속 5균주에 대한 염기서열을 분석한 결과, 이들 속은 크게 두 개의 군으로 분류되었다. 첫 번째 분류군은 aGARICUS BLAZEI atcc 76739, 현재 우리나라에서 배양중인 Agaricus blazei, agaricus arvensis IMSNU 32049, 그리고 Agaricus campestris VPI-OKM 25665로 이루어 졌다. 특히, Agaricus blazei ATCC 76739와 현재 우리나라 농가에서 배양중인 Agaricus blazeisms ITS 부위에서 5개의 염기서열에서 변이가 발견되었다. 이러한 변이는 지리적 또는 배양상의 변이로 인하여 특정염기서열에서 변이가 발생한 것으로 간주되므로 이들은 상호 동일종일 것으로 추정된다. 또한, Agaricus arvensis IMSNU 32049 와 Agaricus ampestris VPI-LKM 25665는 Agaricus blazei 하위 분류군을 형성하였다. 두 번째 분류군은 Agaricus bisporus CH 3004와 Agaricus pocillator DUKE-J 173 으로 이루어졌다.

Molecular spslemaucs of Agaricus species was investigated on the base of the sequences of the internal transcribed spaceriITS) regions in ribosomal DNA (rDNA). The sequences of the ITS region in 5 species and two group of Agaricus genus were resolved. In the phylogenetic trees. the species generally divided inlo two subclusters, refered to here as the group I and group 11. The group I consisted of Agaricus blazei ATCC 76739, Agarictrs blazei species cultivated in Korean hmings. Ago/-icus anmensis IMSNU 32049 and Agaricus can~pestris VPI-OKM 25665. Between Agaricus blazei NCC 76739 and the Agaricus blazei species cultivated in Korean farmings had the variation in lhe 5 nucleotide on the ITS regions. These varieties were presumed the variation by the geographic and cultivated conditions. In addition the subgroup of group I was formed by Agaricus arvensis LMSNU 32049 and Agaricus carnpests VPI-OKM 25665. The group IT included Agnrictrs bispoms CH 3004 and Agaricus pocillotor DUKE-J 173.

키워드

참고문헌

  1. Nucl. Aci. Res. v.21 Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride Heng, Z.;F. Qu;L.H. Zhu.
  2. Nippon Yakurigaku Zasshi v.110 A stable culturing method and pharmacological effects of the Agaricus blazei Higaki, M.;F. Eguchi;Y. Watanabe
  3. In mushroom Science XⅢ v.1 A new systems for identifying cultures for Ganoderma species Hseu, R.S.;H.H. Wang;Michael J. Maher(ed.)
  4. Mammalinan Protein Metabolism Evolution of protein molecules Juke, T.H.;C.R. Cantor; In H. N. Munro(ed.)
  5. Carbohydr. Res. v.186 no.2 Fractionation and antitumor activity of the water-insoluble residue of Agaricuc blazei fruiting bodies Kawagishi, H.;R. Inagake;T. Mizuno;K. Shimura;H. Ito;T. Hagiwara;T. Nakamura
  6. Mycologist v.9 no.2 Sequence or Structure?/A short review on the application of nucleic acid sequence information to fungal taxonomy Michell, J.I.;P.J. Robert;S.T.Moss
  7. Biosci. Biotechnol. Biochem. v.62 no.3 Polysaccharides from Agaricus blazei stimulate lymphocyte T-cell subsets in mice Mizuno, M.;M. Morimoto;K. Minato;H. Tsuchida
  8. Yakugaku Zasshi v.114 no.5 Antimutagenic and bactericidal substances in the fruit body of a Basidiomycete Agaricus blazei Osaki, Y.;T. Kato;K. Amamoto;J. Kubo;T. Mizaki
  9. RDA. J. Agri. Sci. v.36 no.1 Analysis of interspecific allozyme variation within the genus Ganoderma by polyacrylamide gel isoelectric focusing Park, D.S.;J.M. Sung;Y.S. Kim;Y.B.Yoo;Y.J. Ryu;D.Y.Cha
  10. Mushroom: Agaricus blazei Park, S.G.;Y.G. Park;S.G. Choi
  11. Mol. Bio. Evol. v.24 The neighbor-joining method: A new method for reconstructing phylogenetic tress. Saitou, N.;M. Nei
  12. Nucl. Aci. Res. v.22 Clustal W: improving the sensitivity of progressive multiple sequences alignment through sequence weighting, position-specific gap penalties and weight matrix choice Thomson, J.D.;D.G. Higgins;T.J. Gibson
  13. PCR protocols: a guide to methods and application Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics White, T.;J. Burns;S. Lee;J. Taylor;Innis, M.A.D.H.Gelfand;J.J. Sninsky;T.J. White(eds.)